Electronic Resource
Springer
Plant cell, tissue and organ culture
37 (1994), S. 197-199
ISSN:
1573-5044
Keywords:
Apomixis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Hieracium aurantiacum L. is being investigated as a model system for studying the genetic basis of gametophytic apomixis. Micropropagation methods were developed for the multiplication and maintenance of unique genotypes, and to facilitate the development of an Agrobacterium-mediated transformation procedure. A basal medium containing Murashige & Skoog macro- and micro-salts, B5 vitamins and 3% sucrose was used throughout. Shoot regeneration from leaf disks was optimized on a medium supplemented with 2.5 μM indole-3-butyric acid (IBA) and 2.2 μM 6-benzyladenine (BA). Shoot proliferation was optimized using 2.2 μM BA, without additional IBA, giving 5-fold shoot-tip multiplication rates every 4 weeks. Shoots readily formed roots on the basal medium and could be transplanted to potting medium 3 weeks later. Micropropagation was found to have no effect on the expression of apomixis in this species.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043616
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