ISSN:
1612-1112
Schlagwort(e):
Chemical excitation source
;
Chemiluminescence detector
;
Chemical band narrowing
;
Dansyl derivative
;
Serum
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary A peroxyoxalate chemiluminescence detection system for liquid chromatography is described. The excitation of fluorophores is generated by the reaction of bis-(2,4,6-trichlorophenyl)oxalate or bis(2,4-dinitrophenyl)oxalate and hydrogen peroxide, which are added to the column effluent. The influence of the solvents and the concentrations of the reagents have been investigated. The influence of the flow cell volume on sensitivity and on band broadening have also been studied and a “chemical band narrowing effect” has been observed. Different types of apparatus have been compared for detection of the emitted light. The system has been used for the detection of the dansyl derivative of a drug with a secondary amine functional group in serum samples. The detection limits are in the 1–10pg range.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02258768
Permalink
