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  • 1
    Electronic Resource
    Electronic Resource
    The Sulfolobus-“Caldariella” group: Taxonomy on the basis of the structure of DNA-dependent RNA polymerases (1980)
    Springer
    Archives of microbiology 125 (1980), S. 259-269 
    Details
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Caldariella ; DNA-dependent RNA polymerase ; Sulfolobus ; Taxonomy ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The similarity of the morphology and of DNA composition, the homology of the component patterns of DNA-dependent RNA polymerases and their immunochemical crossreactivity support the conclusion that several extreme thermoacidophiles are related to each other. We name two new species of the genus Sulfolobus. The first, Sulfolobus solfataricus (DSM 1616 and DSM 1617) has the same GC content in its DNA and the same general properties as S. acidocaldarius, but differs significantly from the latter species in the molecular weights of the 11 components of its RNA polymerase and in the salt requirements of this enzyme. The second, Sulfolobus brierleyi, DSM 1651, differs from S. acidocaldarius in several respects. The cells show much less stability at neutral pH. The GC content is significantly lower. The RNA polymerase lacks two components present in the enzymes from the other species. The residual 9 components show larger size differences from the homologous subunits of the S. acidocaldarius enzyme. Like the enzyme from S. solfataricus, the polymerase from S. brierleyi yields an incomplete immunochemical crossreaction with an antibody against the RNA polymerase from S. acidocaldarius. The isolates DSM 1616 and DSM 1617 of Sulfolobus solfataricus are probably identical with or similar to the “Caldariella” strains MT 3 and MT 4, isolated by de Rosa et al. (1975). Like all other known archaebacterial RNA polymerases the enzymes from these species are insensitive to rifampicin and streptolydigin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446886
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  • 2
    Electronic Resource
    Electronic Resource
    Methanoplanus limicola, a plate-shaped methanogen representing a novel family, the methanoplanaceae (1982)
    Springer
    Archives of microbiology 132 (1982), S. 31-36 
    Details
    ISSN: 1432-072X
    Keywords: Methanogens ; Archaebacteria ; Cell division ; Glycoprotein ; Acetate ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An angular plate-shaped weakly motile mesophilic methanogen was isolated from a swamp of drilling waste in Italy. Growth occurs on H2/CO2 or on formate. Acetate is required in addition. The optimal doubling time is 7 h at 40° C. The cell envelope is composed most likely of glycoprotein subunits in hexagonal arrangement. The GC-content of its DNA is 47.5 mol%. On the basis of DNA-RNA hybridization it was found to represent a new family, the Methanoplanaceae within the order Methanomicrobiales.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690813
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  • 3
    Electronic Resource
    Electronic Resource
    Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C (1986)
    Springer
    Archives of microbiology 145 (1986), S. 56-61 
    Details
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Extremely thermophilic ; Marine ; Pyrococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten strains representing a novel genus of marine thermophilic archaebacteria growing at between 70 and 103°C with an optimal growth temperature of 100°C and a doubling time of only 37 min were isolated from geothermally heated marine sediments at the beach of Porto di Levante, Vulcano, Italy. The organisms are spherical-shaped, 0.8 to 2.5 μm in width and exhibit monopolar polytrichous flagellation. They are strictly anaerobic heterotrophs, growing on starch, maltose, peptone and complex organic substrates. Only CO2 and H2 could be detected as metabolic products, the latter being inhibitory to growth at high concentrations. Hydrogen inhibition can be prevented by the addition of So, whereupon H2S is formed in addition, most likely as the result of a “detoxification” reaction. The GC-content of the DNA of isolate Vc 1 is 38 mol%. The new genus is named Pyrococcus, the “fireball”. Type species and strain is Pyrococcus furiosus Vc 1 (DSM 3638).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413027
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  • 4
    Electronic Resource
    Electronic Resource
    Methanococcus thermolithotrophicus, a novel thermophilic lithotrophic methanogen (1982)
    Springer
    Archives of microbiology 132 (1982), S. 47-50 
    Details
    ISSN: 1432-072X
    Keywords: Methanogens ; Archaebacteria ; Autotrophic ; Thermophilic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An autotrophic thermophilic motile coccoid methanogen was isolated from geothermally heated sea sediments close to Naples, Italy. Growth occurs on H2/CO2 and on formate between 30 and 70°C with an optimum at 65°C. The optimal doubling time is only 55 min. The NaCl-concentration ranges from 1.3% to 8.3% with an optimum around 4%. By its G+C-content of 31.3 mol%, its subunit envelope, and by DNA-RNA hybridization the new isolate is clearly defined to be a member of the genusMethanococcus. We name itMethanococcus thermolithotrophicus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690816
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  • 5
    Electronic Resource
    Electronic Resource
    Thermococcus litoralis sp. nov.: A new species of extremely thermophilic marine archaebacteria (1990)
    Springer
    Archives of microbiology 153 (1990), S. 205-207 
    Details
    ISSN: 1432-072X
    Keywords: Archaebacteria ; Extreme thermophile ; Pyrococcus ; Thermococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a new species, Thermococcus litoralis, which is different from the type species Thermococcus celer in molecular, morphological and physiological characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247822
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  • 6
    Electronic Resource
    Electronic Resource
    Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110°C (1991)
    Springer
    Archives of microbiology 156 (1991), S. 239-247 
    Details
    ISSN: 1432-072X
    Keywords: Methanopyrus ; Methanogens ; Archaea ; Hyperthermophilic ; Marine ; Vents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel group of hyperthermophilic rod-shaped motile methanogens was isolated from a hydrothermally heated deep sea sediment (Guaymas Basin, Gulf of California) and from a shallow marine hydrothermal system (Kolbeinsey ridge, Iceland). The grew between 84 and 110°C (opt: 98°C) and from 0.2% to 4% NaCl (opt. 2%) and pH 5.5 to 7 (opt: 6.5). The isolates were obligate chemolithoautotrophes using H2/CO2 as energy and carbon sources. In the presence of sulfur, H2S was formed and cells tended to lyse. The cell wall consisted of a new type of pseudomurein containing ornithin in addition to lysine and no N-acetylglucosamine. The pseudomurein layer was covered by a detergent-sensitive protein surface layer. The core lipid consisted exclusively of phytanyl diether. The GC content of the DNA was 60 mol%. By 16S rRNA comparisons the new organisms were not related to any of the three methanogenic lineages. Based on the physiological and molecular properties of the new isolates, we describe here a new genus, which we name Methanopyrus (the “methane fire”). The type species is Methanopyrus kandleri (type strain: AV19; DSM 6324).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262992
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