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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 62 (1984), S. 1102-1107 
    ISSN: 1432-1440
    Keywords: Anorexia ; Aromatic amino acids ; Branched-chain amino acids ; Dopaminergic neurons ; Neoplastic diseases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Studies in anorectic tumor-bearing rats indicate that anorexia is correlated to imbalances of neutral amino acids in blood and CNS. Consequently plasma amino acids of patients with neoplastic and non-neoplastic internal diseases were studied during phases of anorexia; special regard was given to the precursors of dopamine and serotonin. Anorectic patients were compared to non-anorectic patients with neoplasia. During anorexia, plasma levels of valine and leucine and hence the ratio of the molar concentrations of Val+Leu+Ile/Phe+Tyr were significantly decreased in each anorectic patient as compared to non-anorectic patients whose ratios were always within the normal ranges. As aromatic and branched-chain amino acids compete for penetration of the blood brain barrier, the decrease of the amino acid ratio may induce a raised flux of phenylalanine and tyrosine into the CNS which results in an increased activation of dopaminergic neurons — known to cause anorexia.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 168 (1986), S. 101-105 
    ISSN: 1432-2048
    Keywords: Catharanthus (cell cycle) ; Cell cycle ; Glycoprotein ; Nicotiana (cell cycle) ; Tunicamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of tunicamycin, an inhibitor of N-glycosylation of proteins, on growth and on synthesis of DNA and protein was studied in suspension cultures from Nicotiana tabacum and Catharanthus rosea. In the presence of 0.1–1 μg · ml-1 tunicamycin, cell division and DNA synthesis stopped in cells which had been proliferating logarithmically, but protein formation continued. Cytophotometric determination of the nuclear DNA content in Catharanthus cells showed that a cell-cycle arrest had occurred in G1 phase. Metabolic labelling of cells with the glycoprotein precursors glucosamine or mannose was inhibited, too. The results indicate that one or more glycoproteins are needed for the cell to pass through the G1 phase, as was recently postulated for animal and yeast cells.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 307 (1981), S. 404-408 
    ISSN: 1618-2650
    Keywords: Ionenselektive Disk-Elektrode ; Chloridselektive Elektrode ; Chloridbestimmung im Blut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Messung von Chlorid im heparinisierten Blut mit einer ionenselektiven Disk-Elektrode im strömungsoptimierten Durchflußsystem wird vergleichend anderen etablierten Meßverfahren der klinischen Chemie für Cl−-Messungen im Serum, wie Coulometrie und Mercurimetrie, gegenübergestellt. Eine gute Korrelation dieser Methoden wird demonstriert. Die direkte Blutmessung von Cl− vermeidet Meßwertverfälschungen durch Probenaufbereitung oder wie sie bei Messungen mit ionenselektiven Kristallmembranelektroden in Serumproben durch lange Stehzeiten auftreten können. Die Kalibrierung erfolgt durch wäßrige NaCl-Lösungen unter Hydrogencarbonatzusatz. Meßkurven dokumentieren die Potentialstabilität.
    Notes: Summary The measurement of chloride in heparinized blood by means of an ion-selective disc electrode in a flow-optimized flow-through system is compared with other established methods in clinical chemistry for Cl− measurements in serum, e.g. coulometry and mercurimetry. The methods are shown to correlate well. The direct blood measurement of Cl− prevents falsification of data due to sample preparation or due to long retention periods such as may occur in serum samples with measurements using ion-selective crystal membrane electrodes. Calibration is effected by means of aqueous solutions of NaCl under addition of hydrogencarbonate. Graphs prove the stability of potential.
    Type of Medium: Electronic Resource
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