ISSN:
1476-5535
Keywords:
Biocatalysis
;
Non-conventional media
;
Aromatic hydroxylation
;
Dioxygenation
;
Reactor operation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Application of biotransformations to the synthesis of industrial chemicals is in part limited by a number of process challenges. We discuss the conversion of toxic, poorly water-soluble organic substrates by whole cells, using as an illustrative example the specific hydroxylation of toluene to toluenecis-glycol byPseudomonas putida UV4. Toxic effects may be eliminated through the introduction of tetradecane, to partition toluene away from the biocatalyst, to give product concentrations of 30–60 gL−1, in a two-liquid-phase reactor. The operational limits of this system have been experimentally determined and are presented in the form of windows of operation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01569955
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