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  • 1
    Electronic Resource
    Electronic Resource
    Ri-mediated transformation of Artemisia annua with a recombinant farnesyl diphosphate synthase gene for artemisinin production (1999)
    Springer
    Plant cell, tissue and organ culture 57 (1999), S. 157-162 
    Details
    ISSN: 1573-5044
    Keywords: Artemisia annua ; artemisinin ; farnesyl diphosphate synthase ; hairy roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A transgenic system was developed for Artemisia annua L. via Agrobacterium rhizogenes-mediated transformation. Using this system a cDNA encoding farnesyl diphosphate synthase (FDS) placed under a CaMV 35S promoter was transferred into Artemisia annua using Agrobacterium rhizogenes strain ATCC15834. Among the 150 hairy root lines established, 16 lines showed resistance to kanamycin (20 mg l-1). The intergration of FDS gene was confirmed by PCR and Southern blot analysis, and analysis of Northern blot revealed that the foreign FDS gene was expressed at the transcriptional level in three hairy root lines (F-1, F-24 and F-26 root line). F-1, F-24 and F-26 root lines grew faster than the control hairy root line. However, on the MS medium growth of F-26 root line was abnormal in that callus frequently formed. Analysis of artemisinin demonstrated that about 2–3 mg g-1 DW of artemisinin were then detected in the three root lines, which is about 3–4 times higher than that in the control hairy roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006326818509
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  • 2
    Electronic Resource
    Electronic Resource
    Use of parameter estimation in determining soil hydraulic properties from unsaturated transient flow (1990)
    Springer
    Water resources management 4 (1990), S. 1-19 
    Details
    ISSN: 1573-1650
    Keywords: Soil hydraulic properties ; unsaturation ; parameter estimation ; cumulative drainages ; head profiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: Abstract This paper proposes a model for determining the parameters given by the closed-form equations of van Genuchten. An objective function is made by the observed data from vertical drainage, and the solutions of optimization show that less computation and more accurate estimates are made as head profiles are taken into account rather than cumulative drainage. Sensitivity analysis of the error vector to parameters interprets this reason. The convergence and stability of solutions are evaluated with different magnitudes of measured errors in the head, and the results show good estimates will be obtained if a sufficient pressure head at the soil bottom is applied. A variable θ k is introduced to avoid the estimations of α and n being affected by the uncertainties of Ks and θ s .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00429922
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genomic organization of an α-zein gene cluster in maize (1992)
    Springer
    Molecular genetics and genomics 231 (1992), S. 304-312 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; Zein ; Gene cluster ; Repetitive sequence ; Cos mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes encoding the α-zein proteins of maize constitute a large multigene family of some 75 genes. This multigene family can be divided into four subfamilies based on the nucleotide sequences of their genes and the deduced amino acid sequences of their proteins. We describe for the first time evidence of a clustering of five α-zein subfamily 4 (SF4) genes that are members of one of the major α-zein subfamilies in a 56 kb region of the genome of the maize inbred line W22. None of the other three known α-zein gene subfamilies (SF1, SF2, or SF3) are present in this cluster. The genomic region was reconstructed using restriction endonuclease maps to identify and align three overlapping cosmid clones isolated from a genomic library. The α-zein genes are not evenly spaced; the minimum distance between genes is 3.5 kb; the maximum is 13 kb. All the α-zein genes in the cluster have the same transcriptional orientation. The location and sequences of some of the repetitive DNA elements in this gene cluster were determined. We estimate that there are a minimum of eight repetitive DNA elements in this region. The sequences of the repetitive elements (not functionally defined) are located between or among the α-zein genes. The regions containing two of these repetitive elements (Rep1 and Rep4) have been sequenced; they are about 15 kb apart in the genome. These repetitive elements have similar sequences for about 300 by out of the 400 by compared. The regions of sequence similarity, however, are in reverse orientation to one another. Both repetitive elements contain replication origin-like sequences. In addition, Rep4 contains two repeats of a five-base sequence that appear to define its presumptive ends. The presence of the short direct repeats flanking the Rep4 element suggests that the Rep4 element might have originated from a transposition event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279804
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    Paper (German National Licenses)
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