ISSN:
1434-9949
Keywords:
Rheumatoid Arthritis
;
Cell Membrane
;
Polyacrylamide Gel Electrophoresis
;
Membrane Proteins
;
Surface Labelling NaB3H4
;
Synovitis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Fibroblast cultures were started from synovial tissue samples of 12 rheumatoid arthritic, 9 non-rheumatoid synovitic and 6 control patients. External galactose units of plasma membrane glycoproteins of confluent cells were labelled using the galactose oxidase-tritiated borohydride method. These surface-labelled cells were analyzed for possible differences in their glycoproteins by electrophoresis in SDS-containing polyacrylamide gradient gels. Total cell lysates were separated into 50–60 polypeptide bands. Fluorography of the gels revealed about 20 labelled plasma membrane glycoproteins. Some strain-specific differences were detected between the samples in all the groups, but no correlation with rheumatoid arthritis could be demonstrated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02032172
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