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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 36 (1977), S. 207-213 
    ISSN: 1439-6327
    Keywords: Anaerobic training ; Human skeletal muscle ; Phosphagens ; LDH ; Anaerobic capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of a 6 week program of intense, intermittent hill running was investigated in 5 endurance-trained men (34–37 years). Venous lactate responses averaged 10.7 mM×1−1 during training. Biopsies were obtained from the vastus lateralis before and after training for determinations of histochemical classification, phosphagen concentrations (ATP and CP) and activities of total LDH and selected LDH isozymes. Indices of aerobic and anaerobic capacity were also measured. Significant improvements (P〈0.01) were noted in training distance and loads employed in leg presses, also part of the training: the treadmill test for anaerobic capacity improved by 16.7% and the terminal blood lactate level increased 14% (P〈0.05). No changes occurred in $$\dot V$$ O2 max, body fat per cent or anaerobic power. Muscle ATP concentration increased by 14.8% (P〈0.01) whereas the tissue level of CP remained unchanged. Total LDH activity and the LDH isozymes (LDH-1, LDH-5 and LDH-2, 3, 4) were not altered by training. No changes in fibre distribution were observed. That adaptations to the anaerobic training did occur is evident, but the cellular locus remains to be elucidated further.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Key wordsClostridium ; Aryl-β-glucoside ; PTS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A phosphotransferase-dependent aryl-β-glucoside uptake and utilisation system (abg) was isolated from the ruminal Clostridium (“C. longisporum”). The system is composed of three genes, abgG, abgF and abgA, and a number of regulatory regions, including terminator/antiterminator type stem-loop structures preceding the abgG and abgF genes. Similarity analysis of the proteins encoded by these genes indicated that they were responsible for the regulation of the abg system through antitermination (AbgG), the uptake and phosphorylation of aryl-β-glucosides (AbgF) and the hydrolysis of the intracellular phosphorylated glycosides (AbgA). Experimental evidence for the functions of AbgF and AbgA was obtained. Although it was not possible to demonstrate any function for AbgG, a promoter 5′ to the abgG gene was identified which was responsible for expression of the downstream genes. The abg system is remarkably similar to operons from the gram negative Enterobacteriaceae, both in the coding and non-coding regulatory regions.
    Type of Medium: Electronic Resource
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