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  • 1
    Electronic Resource
    Electronic Resource
    Detection and localisation of HIV-1 DNA and RNA in fixed adult AIDS brain by polymerase chain reaction/ in situ hybridisation technique (1999)
    Springer
    Acta neuropathologica 98 (1999), S. 481-487 
    Details
    ISSN: 1432-0533
    Keywords: Key words AIDS ; HIV encephalitis ; HIV-1 DNA and ; RNA ; Polymerase chain reaction-in situ (PCR-IS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the brain of patients with AIDS, HIV-1 is localised in a productive form in mononuclear cells. One issue that still needs clarification is whether HIV is localised in cells other than those of mononuclear lineage. Gene amplification by polymerase chain reaction/in situ hybridisation (PCR-IS) could shed light on it. In this study, formalin-fixed, paraffin-embedded brain tissue from ten adult AIDS sufferers was used. Five of them showed evidence of HIV encephalitis (HIVE), five did not show any abnormality. Nested PCR revealed HIV-1 DNA in all HIVE cases and in three of the group without HIVE. HIV-1 DNA and RNA were also detected in situ in seven cases (all seven were also HIV-1 DNA positive in tube). A higher signal was located in the white than in the grey matter. HIV-1 DNA was found in microglia, macrophages, perivascular cells, multinucleated gaint cells (MGC) and in CD68-negative cells. Some of them were identified as endothelial cells, astrocytes and oligodendrocytes. Reverse transcriptase-PCR-IS was positive in macrophages, MGC, endothelial and glial cells. These results confirm infection of endothelial cells and other glial cells and give clues about the route of entry of virus into the central nervous system and the pathogenesis of the disease. This study did not give any convincing evidence supporting an infection of neurons by HIV-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010051113
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The ultrastructure of septal pores and associated structures in the ascogenous hyphae and asci ofSordaria humana (1981)
    Springer
    Protoplasma 107 (1981), S. 127-147 
    Details
    ISSN: 1615-6102
    Keywords: Asci ; Ascomycetes ; Ascogenous hyphae ; Croziers ; Intercellular communication ; Septal pores ; Sordaria humana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Septal pores and associated structures have been studied in ascogenous hyphae, croziers and asci ofSordaria humana by means of electron microscopy of serial and random sections. Pores exhibit variable structures from relatively simple pore caps to complex swollen rims with associated membrane cisternae. The simple types are found at the base of the ascogenous hyphae while the complex forms occur at the apex, in the croziers and in very young, presporulation asci. Post-sporulation asci contain a relatively simple type of pore structure. Cells which subtend the ascogenous hyphae exhibit both open and capped pores in their cross walls. Pore structures may be asymmetric in which case they show greater complexity on the side of the cross wall nearest to the apex or crozier. Membranous components of the complex pores are continuous with the endomembrane systems of the two adjacent cells and thus with the outer membranes of the nuclear envelopes. Membrane continuities may connect prefusion nuclei or fusion nuclei in penultimate cells, with nuclei of the stalk and terminal cells of croziers. Some speculation is presented as to the implication and possible roles of these structures in relation to cell differentiation within the ascogenous hyphae and croziers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275613
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Uromyces viciae-fabae onVicia faba: Scanning electron microscopy of frozen-hydrated material (1982)
    Springer
    Protoplasma 111 (1982), S. 28-37 
    Details
    ISSN: 1615-6102
    Keywords: Frozen-hydrated ; Low temperature SEM ; Uredium ultrastructure ; Uromyces viciae-fabae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructure of uredia, intercellular and intracellular hyphae ofUromyces viciae-fabae onVicia faba has been studied by scanning electron microscopy of frozen-hydrated material. Frozen-hydrated leaves will readily fracture and can be viewed on the cold stage of the microscope at liquid nitrogen temperatures for several hours without visible surface changes occurring. Both fungal and host-plant cells show a high degree of morphological preservation with little or no loss of cell contents and with minimal signs of shrinkage. Intracellular hyphae may be filamentous and branched or short and lobed. They are constricted at the point of entry into the host cell. Intercellular matrix material is well preserved around developing pedicels and urediospores and may act as a lubricant. The technique described is very quick compared with conventional preparatory procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287644
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    Paper (German National Licenses)
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