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An aureobasidin A resistance gene isolated from Aspergillus is a homolog of yeast AUR1, a gene responsible for inositol phosphorylceramide (IPC) synthase activity (1999)

Kuroda, M. ; Hashida-Okado, T. ; Yasumoto, R. ; [et al.]

Springer

Molecular genetics and genomics 261 (1999), S. 290-296

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ISSN: 1617-4623

Keywords: Key words Aureobasidin A ; Aspergillus nidulans ; Aspergillus fumigatus ; Drug-resistant mutant ; aurA

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The AUR1 gene of Saccharomyces cerevisiae, mutations in which confer resistance to the antibiotic aureobasidin A, is necessary for inositol phosphorylceramide (IPC) synthase activity. We report the molecular cloning and characterization of the Aspergillus nidulans aurA gene, which is homologous to AUR1. A single point mutation in the aurA gene of A. nidulans confers a high level of resistance to aureobasidin A. The A. nidulans aurA gene was used to identify its homologs in other Aspergillus species, including A. fumigatus, A. niger, and A. oryzae. The deduced amino acid sequence of an aurA homolog from the pathogenic fungus A. fumigatus showed 87% identity to that of A. nidulans. The AurA proteins of A. nidulans and A. fumigatus shared common characteristics in primary structure, including sequence, hydropathy profile, and N-glycosylation sites, with their S. cerevisiae, Schizosaccharomyces pombe, and Candida albicans counterparts. These results suggest that the aureobasidin resistance gene is conserved evolutionarily in various fungi.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004380050969

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Guided bone tissue elaboration by osteogenic cells in vitro (1993)

Gomi, K. ; Davies, J. E.

Hoboken, NJ : Wiley-Blackwell

Journal of Biomedical Materials Research 27 (1993), S. 429-431

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ISSN: 0021-9304

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine , Technology

Notes: Primary rat bone marrow cells were cultured for 2 weeks in polystyrene dishes whose surfaces had been roughened using 600- or 320-grit silicon carbide paper. Eight samples were prepared of each of the three groups of dishes, to include a nontreated control suface. Following the culture period, the dishes were stained by von Kossa's method. The distribution of bone formed during the culture period was examined by light microscopy and the area of bone formed quantified. Results demonstrated that both the amount and spatial distribution of bone were influenced by the roughness of the underlying substratum. Differences between the smooth and roughened surfaces were statistically different at P 〈 .05. © 1993 John Wiley & Sons, Inc.

Additional Material: 2 Ill.