ISSN:
1617-4623
Keywords:
Aspergillus nidulans
;
Transformation
;
Deletions
;
Heterologous integration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The analysis of four transformants for the proline catabolism (prn) gene cluster of Aspergillus nidulans is reported. Using a combination of traditional genetic methodology and Southern hybridisation we have shown that in two cases multiple copies of the transforming plasmid have been integrated into linkage groups other than VII, which contains the prn cluster. In the other two cases integration of the plasmid has probably occurred homologously. The phenotype of these transformants is broadly consistent with increased copy number resulting in increased expression. Genetic manipulation of these transformants using the sexual or parasexual cycles has shown that recombination events during and possibly also subsequent to integration of the transforming DNA can generate new mutational lesions, in particular, deletions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00422084
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