ISSN:
1573-4846
Keywords:
itaconic acid
;
biosynthesis
;
Aspergillus terreus
;
fungi
;
aconitate decarboxylase
;
E.C. 4.1.1.6
;
enzyme immobilization
;
silicate
;
sol-gel
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new technique of investigation of labile metabolic pathways using immobilization of the pathway in sol-gel derived silicate matrices was demonstrated. The biosynthetic pathway of itaconic acid in Aspergillus terreus is believed to proceed through decarboxylation of cis-aconitate catalyzed by an unstable enzyme aconitate decarboxylase, E.C. 4.1.1.6. Stabilization of this pathway in sol-gel derived silicate matrix enabled the elucidation of the correct sequence of biosynthetic steps. The decarboxylation of cis-aconitate does not lead directly to itaconate, but rather to citraconate. The latter is then isomerized to itaconate by a previously unknown enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00401893
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