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  • Assembly  (1)
  • Azathioprine  (1)
  • Cannabinoids, enzyme immunoassay  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 1187 (1994), S. 260-263 
    ISSN: 0005-2728
    Keywords: Assembly ; Cytochrome c ; Heme synthesis ; Heme/copper oxidase ; Membrane transport ; Periplasm
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International journal of legal medicine 94 (1985), S. 219-225 
    ISSN: 1437-1596
    Keywords: Cannabinoids, enzyme immunoassay ; THC-metabolite, detection in urine and blood ; Cannabinoide, Enzymimmunoassay ; THC-Metabolit, Nachweis in Urin und Blut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Mit Hilfe des Enzymimmunoassay (EMIT-dau-Syva) lassen sich Cannabinoide in Blut und Urin nach inhalativer (10 mg THC) oder oraler (30 mg THC) Aufnahme nachweisen. Die Kurvenverläufe werden mitgeteilt. Im Urin waren erhöhte Spiegel bis zu einer Woche nachweisbar. Eine Absicherung der EMIT-Befunde ist mittels Dünnschichtchromatographie nach absorptiver Anreicherung an einer C18-Extraktionssäule möglich. Nachweisgrenze ist 10 ng THC-Carbonsäure/ml bei Einsatz von 10 ml Urin.
    Notes: Summary The EMIT cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in urine remain detectable up to 1 week. Confirmation was done by adsorption of the THC carboxylic acid onto a C18 extraction column and elution with acetone and TLC. The method is simple and sensitive and is applicable with common laboratory equipment. The detection limit is 10 ng/ml, using 10 ml urine.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 181 (1982), S. 19-25 
    ISSN: 1433-8580
    Keywords: Gas chromatography ; Serum level ; Azathioprine ; 6-Mercaptopurine ; Immunosuppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The knowledge of serum levels of azathioprine or its metabolite 6-mercaptopurine is important for the monitoring of the immunosuppressive therapy after kidney transplantation, especially when kidney function is low. For this reason, the work of Bailey et al. was modified. 6-Mercaptopurine was extracted from the serum with a mixture of isopropanol-ethylacetate (1:1 v:v), derivatized with trimethylaniliniumhydroxyde and measured gaschromatographically. Sulfhydryl-protecting reagents were added to prevent loss of substance. Oxyethyltheophylline was used as the internal standard. The extraction and clean-up method recovered 80% of the 6-mercaptopurine present, the lower limit of sensitivity was 20 ng/ml, i.e., ten times lower than that of Bailey et al. The method could be realized on eight patients treated with azathioprine, the half life of the drug was about 20–40 min within the first hour after i.v. application.
    Type of Medium: Electronic Resource
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