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T-DNA-tagged chromosome 12 in donor Lycopersicon esculentum × L. pennellii is retained in asymmetric somatic hybrids with recipient Solanum lycopersicoides (1993)

McCabe, P. F. ; Dunbar, L. J. ; Guri, A. ; [et al.]

Springer

Theoretical and applied genetics 86 (1993), S. 377-382

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ISSN: 1432-2242

Keywords: Asymmetric hybridization ; Cell fusion ; Lycopersicon esculentum × L. pennellii-Solanum lycopersicoides ; T-DNA tag

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Asymmetric somatic hybrid plants were recovered after fusing irradiated mesophyll protoplasts of donor Lycopersicon esculentum × L. pennellii (EP) interspecific hybrid with callus-derived protoplasts of recipient Solanum lycopersicoides. EP plant A54 had been previously transformed by an agrobacterium vector, and the T-DNA insert mapped to the L. esculentum chromosome 12. The T-DNA insert conferred kanamycin resistance to EP that was subsequently used to select cell fusion products and recover asymmetric hybrid plants that retained tagged chromosome 12. Doses of 50- and 100-Gy irradiation promoted the elimination of only a few donor chromosomes. At 200 Gy, the regenerated plants had ploidy levels higher than tetraploid. However, the T-DNA tagged chromosome 12 was always retained in the asymmetric hybrid plants tested. Likewise, all plants from the 100-Gy series, with the exception of number 160, were mixoploid in the root-tip cells. Such mixoploid asymmetric somatic hybrids could be stabilized by inducing adventitious shoots on leaf strips cultured on shoot regeneration medium containing kanamycin. The asymmetric hybrid plants did not produce viable seed when self-pollinated or backcrossed to tomato or S. lycopersicoides.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00222105

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Thermal behavior of new polymer electrolytes (1995)

Fauteux, D. ; McCabe, P.

New York, NY : Wiley-Blackwell

Polymers for Advanced Technologies 6 (1995), S. 83-90

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ISSN: 1042-7147

Keywords: polymer electrolytes ; salt ; phase diagram ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Solid polymer electrolytes (SPE) have been identified as a class of materials which could enable the fabrication of high energy density solid state lithium rechargeable batteries which could meet the performance requirements for advanced portable electronic and automotive applications. In order to achieve this goal, novel SPE systems having high ionic conductivity and good mechanical properties at or near ambient temperature must be developed. Novel lithium salts believed to be useful in realizing this objective have recently been proposed. The thermal behavior of SPE systems based on high molecular weight poly(ethylene oxide) (PEO) and on two novel salts, the lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) and the lithium tris(trifluoromethylsulfonyl)-methanide (LiTSFM) is reported and compared with the thermal behavior of the high molecular weight PEO-lithium trifluoromethane sulfonate (LiTFLT) SPE system. Phase diagrams for the PEO-LiTFSI and PEO-LiTFSM SPE systems have been established and are discussed in terms of their impact on SPE-based rechargeable lithium battery technologies. The use of a novel plasticizer in conjunction with the PEO-LiTFSI-based SPE system is reported and it is shown how this modifies the thermal behavior of the PEO-LiTFSI SPE system.

Additional Material: 8 Ill.