ISSN:
1573-8671
Keywords:
Atomic force microscopy
;
scanning force microscopy
;
metaphase chromosomes
;
volume
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Computer Science
,
Natural Sciences in General
Notes:
Abstract A procedure for volume estimation based on scanning force microscopy images is applied to the study of banding-induced structural changes of chromosomes. Therefore, metaphase chromosomes were imaged before and after trypsin digestion, and the resulting three-dimensional data sets were used for a determination of the volumes of the imaged structures. The procedure is based on a histogram-based thresholding. The estimated volume is corrected for the background signal using the average background value from the histogram, so that an automated analysis of the images is possible. A first set of experimental data processed according to this approach is presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1023312421502
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