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  • Botrytis allii  (3)
  • Neurospora crassa  (2)
  • Autochemotropism  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Protoplast responses in the epidermis of Allium cepa induced by penetration by Botrytis allii (1988)
    Springer
    Mycopathologia 102 (1988), S. 169-173 
    Details
    ISSN: 1573-0832
    Keywords: Allium cepa ; Botrytis allii ; penetration responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Penetration of Allium cepa epidermal cells (white, yellow, and red varieties) by Botrytis allii induced a response by host protoplasts in normal tissue which was not observed when penetrations were made in protoplast-free host cell walls. Callose and auto-fluorescing substances (possibly phenolic compounds) were located at the penetration sites only in normal host cells containing protoplasts. Lignin tests were negative. Halos were clearly visible in both types of tissue. Autofluorescence was observed at penetration sites in normal cells of all cultivars but general wall background autofluorescence was not observed in white onions. Autofluorescence was generally yellow green and when treated with ammonium hydroxide became green. Treatment with sodium hydroxide abolished autofluorescence. No attempt was made to isolate the autofluorescing material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437400
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  • 2
    Electronic Resource
    Electronic Resource
    Degenerative changes in the epidermal cell wall of Allium cepa caused by Botrytis allii: Loss of dry mass, pectin, and cellulose in halos (1988)
    Springer
    Mycopathologia 101 (1988), S. 47-52 
    Details
    ISSN: 1573-0832
    Keywords: Botrytis allii ; Allium cepa ; halo composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Halos detected using interference microscopy (even- and fringle-field modes with monoand poly-chromatic light) around penetration sites of Botrytis allii in cell walls of normal and protoplast-free outer epidermal tissue of white, yellow, and red onions were alike. Halos in protoplast-free cell walls contained 33% less dry mass than areas of these walls adjacent to halos (quantitative interference miscroscopy with 546 nm light in the even-field mode). Halos were significantly larger in the white onion than in the yellow and red varieties. The loss of cell wall dry mass during the production of halos involved the loss of pectin and cellulose. We infer that this is caused by enzymes released from the pathogen. Cuticle degradation at penetration sites was not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455668
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ethylene is involved in the autochemotropism of Phycomyces (1977)
    Springer
    Planta 134 (1977), S. 61-66 
    Details
    ISSN: 1432-2048
    Keywords: Autochemotropism ; Avoidance response ; Ethylene ; Phycomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sporangiophore of the fungus Phycomyces blakesleeanus has the property of growing away from a barrier which is few mm from the growing zone of the sporangiophore (avoidance or autochemotropic response). A model has been published (Cohen, R.J., Jan, N.Y., Matricon, J., Delbrück, M.: J. Gen. Physiol. 66, 67–95 (1975)). To explain the avoidance response which postulates that the sporangiophore emits and readsorbs a volatile growth-promoting effector (gas X) and that the barrier modifies the effector distribution by acting as an aerodynamic obstacle, causing a higher concentration of gas X on the side of the sporangiophore closer to the barrier. From this model we deduced three properties of the gas X. Of the several gases tested (N2, CO2, CH4, C2H2, C2H4, C2H6) only ethylene (C2H4) had all these three properties, a finding which suggests that it has a role in the avoidance response (autochemotropism).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390096
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  • 4
    Electronic Resource
    Electronic Resource
    Promoter analysis of the bli-7/eas gene (1993)
    Springer
    Current genetics 24 (1993), S. 394-399 
    Details
    ISSN: 1432-0983
    Keywords: Neurospora crassa ; bli-7, eas, ccg-2 ; Promoter analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between-1498 bp and-1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between-595 bp and-429 bp, as well as a stretch of DNA from-429 bp to-380 bp which may exert a positive influence after light induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351847
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  • 5
    Electronic Resource
    Electronic Resource
    bli-4, a gene that is rapidly induced by blue light, encodes a novel mitochondrial, short-chain alcohol dehydrogenase-like protein inNeurospora crassa (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 223-229 
    Details
    ISSN: 1617-4623
    Keywords: Neurospora crassa ; Blue light ; Mitochondria ; Short-chain alcohol dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Blue light plays an important role in developmental control throughout nature. Thebli-4 gene ofNeurospora crassa, together withbli-3, al-1 andal-2, is rapidly inducible by blue light. Induction leads to a ninety-fold increase in transcription rate over the dark control level, and the gene therefore appears to be of prime importance in the blue-light induction pathway ofN. crassa. We describe the sequencing and analysis ofbli-4 and the 38 kDa protein it encodes. We show that the protein is very rapidly imported into the mitochondria and exhibits high homology with the family of short-chain alcohol dehydrogenases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173767
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  • 6
    Electronic Resource
    Electronic Resource
    Mycelial elongation and sporulation of two fungi on amended media in light or dark (1993)
    Springer
    Antonie van Leeuwenhoek 63 (1993), S. 23-27 
    Details
    ISSN: 1572-9699
    Keywords: 6-benzyladenine ; Botrytis allii ; caffeic acid ; chlorogenic acid ; Colletotrichum dematium ; kinetin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Botrytis allii andCollectotrichum dematium are onion pathogens which can infect in the field and cause decay in storage. Some phenolics can hinder development of these fungi, but the effect of cytokinins is not clear. Cytokinins (kinetin or 6-benzyladenine) or phenolics (caffeic or chlorogenic acids) were added to agar at concentrations of 0 to 10−3 M. Cultures were continuously irradiated with fluorescent light or maintained in the dark for 6 days. On unamended media, final mycelial elongation was 45 or 17.8 mm and sporulation was 28 or 10.6 × 104 spores/ml forBotrytis andColletotrichum, respectively. ForBotrytis, mycelial elongation was slightly (5%) but significantly increased and sporulation increased by 21% by incubation on phenolics as compared to cytokinins. Mycelial extension ofColletotrichum was not affected by amendment. Sporulation ofColletotrichum on kinetin was 16 to 28% greater than on the other amendments. As amendments concentration increased elongation of mycelia of both fungi decreased. Sporulation ofBotrytis increased by 60% as amendment concentration increased from 0 to 10−5 M and then decreased 25% at 10−3 M. As amendment concentration increased from 0 to 10−3 M, sporulation ofColletotrichum increased by 45%. Incubation in light increased mycelial extension 3 to 17% forBotrytis andColletotrichum respectively, and sporulation was increased approximately 78% for both fungi. These compounds do not appear to inhibit development of theseBotrytis orColletotrichum species in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871727
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