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  • Neurospora crassa  (2)
  • Autochemotropism  (1)
  • Cucumis melo  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Planta 134 (1977), S. 61-66 
    ISSN: 1432-2048
    Schlagwort(e): Autochemotropism ; Avoidance response ; Ethylene ; Phycomyces
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The sporangiophore of the fungus Phycomyces blakesleeanus has the property of growing away from a barrier which is few mm from the growing zone of the sporangiophore (avoidance or autochemotropic response). A model has been published (Cohen, R.J., Jan, N.Y., Matricon, J., Delbrück, M.: J. Gen. Physiol. 66, 67–95 (1975)). To explain the avoidance response which postulates that the sporangiophore emits and readsorbs a volatile growth-promoting effector (gas X) and that the barrier modifies the effector distribution by acting as an aerodynamic obstacle, causing a higher concentration of gas X on the side of the sporangiophore closer to the barrier. From this model we deduced three properties of the gas X. Of the several gases tested (N2, CO2, CH4, C2H2, C2H4, C2H6) only ethylene (C2H4) had all these three properties, a finding which suggests that it has a role in the avoidance response (autochemotropism).
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Current genetics 24 (1993), S. 394-399 
    ISSN: 1432-0983
    Schlagwort(e): Neurospora crassa ; bli-7, eas, ccg-2 ; Promoter analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between-1498 bp and-1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between-595 bp and-429 bp, as well as a stretch of DNA from-429 bp to-380 bp which may exert a positive influence after light induction.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1617-4623
    Schlagwort(e): Neurospora crassa ; Blue light ; Mitochondria ; Short-chain alcohol dehydrogenase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Blue light plays an important role in developmental control throughout nature. Thebli-4 gene ofNeurospora crassa, together withbli-3, al-1 andal-2, is rapidly inducible by blue light. Induction leads to a ninety-fold increase in transcription rate over the dark control level, and the gene therefore appears to be of prime importance in the blue-light induction pathway ofN. crassa. We describe the sequencing and analysis ofbli-4 and the 38 kDa protein it encodes. We show that the protein is very rapidly imported into the mitochondria and exhibits high homology with the family of short-chain alcohol dehydrogenases.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Biologia plantarum 43 (2000), S. 373-379 
    ISSN: 1573-8264
    Schlagwort(e): Cucumis melo ; chromatography ; electrophoresis ; host-plant resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An increase in exocarp peroxidase activity was observed in fruit at 5 to 30 days post pollination (DPP), and decreased at 40 and 50 DPP. Total peroxidase activity of the mesocarp was significantly lower than the exocarp in all developmental stages. Mesocarp peroxidase activity decreased consecutively from outer, to middle and, to inner tissue at every developmental stage. Total activity in the mesocarp peaked at 20 DPP. Native-PAGE of exocarp tissue showed at least two cathodic (basic) peroxidases and two anionic (acidic) peroxidases. The number of isozymes was greatest and bands most intense at 30 DPP. IEF-PAGE of the 5 to 50 DPP fruit exocarp showed at least 8 peroxidase isozymes (pI 4.6 to 9.6). Anion exchange chromatography showed only one peak of anionic peroxidase activity that was not evident until 15 DPP. This peak was greatest at 30 DPP and declined at 40 and 50 DPP. Cationic peroxidase isozymes appeared to be the predominant and most intense isoforms throughout fruit development. The changes in peroxidase activity corresponded to fruit formation and may be associated with susceptibility to fruit rot.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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