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  • 1
    Electronic Resource
    Electronic Resource
    Intracellular study of electrical activity of Auerbach's plexus in guinea-pig small intestine (1978)
    Springer
    Pflügers Archiv 374 (1978), S. 265-275 
    Details
    ISSN: 1432-2013
    Keywords: Neurophysiology ; Gastrointestinal function ; Myenteric plexus ; Enteric neurons ; Autonomic nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Intracellular recording revealed two general categories of ganglion cells in Auerbach's plexus. The characteristics of one category were relatively low resting potentials, high input resistance, discharge of spikes throughout a depolarizing current pulse, stimulus-evoked synaptic potentials and spontaneous electrical activity. Characteristics of the second category were high resting potentials, low input resistance, spikes only at the onset of a depolarizing current pulse and long duration hyperpolarizing after-potentials. Responses to extracellular electrical stimulation of the ganglia and interganglionic fiber tracts consisted of electrotonic spread of spikes from the processes to the cell soma, somal action potentials and depolarizing and hyperpolarizing responses that were probably EPSPs and IPSPs. Some of the neurons which received excitatory synaptic input responded with a prolonged train of spikes that outlasted by many seconds the duration of the stimulus to the fiber tract. Spontaneous electrical activity consisted of single EPSPs, patterned bursts of spikes that originated in the cell processes and spread electrotonically to, the recording site, IPSPs and action potentials. The burst-type activity showed periodic conversions from a burst pattern to a trainlike pattern of continuous discharge. Spontaneous discharge of single action potentials was superimposed upon a background of continuous synaptic input to the cell. Spontaneously occurring hyperpolarizing potentials were converted to depolarizing potentials when the membrane was hyperpolarized by current injected through the recording electrode.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585604
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of components of the superoxide generating NADPH oxidase by human leucocytes and other cells (1995)
    Springer
    Protoplasma 184 (1995), S. 79-85 
    Details
    ISSN: 1615-6102
    Keywords: NADPH oxidase ; Superoxide ; Kidney mesangial cell ; Fibroblast ; B lymphocyte ; Endothelial cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary NADPH oxidase of phagocytic leucocytes contains a membrane cytochromeb with two subunits, gp91 phox and p22 phox , together with three cytosolic proteins, p47 phox , p67 phox and p2 rac . The presence of some of these components has been sought in non-phagocytes, using Western blot analysis for protein expression and PCR to amplify and detect mRNA. All components were detected in EBV-transformed B lymphocytes and peripheral blood B lymphocytes. Fibroblasts and human kidney mesangial cells contained mRNA for p67 phox , p47 phox , and p22 phox but not gp91 phox . Levels of expression varied with growth conditions, but it appears possible than an isozyme of cytochromeb which lacks gp9 phox is present in these cells. Proteins of p47 phox and p67 phox were expressed, in low concentrations, in these two cell types. Expression of mRNA for p47 phox and p67 phox was found to be widespread in many cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276904
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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