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  • 1
    Electronic Resource
    Electronic Resource
    An insight in the mechanism of the aminoethylcysteine ketimine autoxidation (1996)
    Springer
    Amino acids 10 (1996), S. 379-390 
    Details
    ISSN: 1438-2199
    Keywords: Amino acids ; Aminoethylcysteine ; Ketimines ; Iron chelates ; Autoxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Oxidation of aminoethylcysteine ketimine (AECK) is followed by the change of 296nm absorbance, by the O2 consumption and by the HPLC analysis of the oxidation products. The oxidation is strongly inhibited by the addition of superoxide dismutase (SOD) but not by hydroxyl radical scavengers or catalase. Addition of EDTA or o-phenanthroline (OPT) favours the oxidation, probably by keeping contaminating metals in solution at the pH studied. Addition of Fe3+ ions strongly accelerates the oxidation in the presence of EDTA or OPT. AECK reacts stoichiometrically with OPT-Fe3+ complex producing the Fe2+ complex which is not reoxidised by bubbling O2. HPLC analyses of the final oxidation products reacting with 2,4-dinitrophenylhydrazine (DNPH) confirm the AECK sulfoxide as the main product of the slow spontaneous oxidation. The detection of other oxidation products when the reaction is speeded up by the addition of the OPT-Fe3+ complex, suggests that the oxidation takes place essentially on the carbon portion of the AECK molecule in the side of the double bond. On the basis of the results presented here, a scheme of reactions is illustrated which starts with the transfer of one electron from AECK to a contaminating metal ion (possibly Fe3+) producing the radical AECK• as the initiator of a self propagating reaction. The radical AECK• reacting with O2 starts a series of reactions accounting for most of the products detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00805865
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Technical improvements for flow karyotyping by standard FACS 440 flow cytometer (1991)
    Springer
    Cytotechnology 5 (1991), S. 195-199 
    Details
    ISSN: 1573-0778
    Keywords: FACS 440 ; FSC signal voltage ; flow karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Chromosomal DNA measurements represent the most accurate methodology for the quantitative analysis of human karyotype. A modified FACS 440 signal collection is described here. The FSC signal voltage coming from the optical bench has been split in two equal parts by a T-connector placed at the preamplifier entrance. The side scatter channel that is not employed during chromosome analysis has been used for the half-FSC signal. This modification allowed the contemporary use of FSC signal both for gating and analysis leading to a better discrimination of background, and therefore to a more accurate definition of sorting windows.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00556289
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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