ISSN:
1432-2048
Keywords:
Auxin (enzyme regulation)
;
Cytokinin (enzyme regulation)
;
β-1,3-Glucanase (hormonal regulation)
;
Nicotiana tabacum
;
Organ specificity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A highly sensitive and specific “rocket” immunoassay was used to measure the content of an endo-type β-1,3-glucanase (EC 3.2.1.39) in tissues of Nicotiana tabacum L. cv. Havana 425. We show that the accumulation of β-1,3-glucanase in cultured pith-parenchyma tissue is blocked by combinations of the auxin, α-naphthaleneacetic acid (NAA), and the cytokinin, kinetin. When tissues pre-incubated for 7 d on complete medium containing 2.0 mg·l-1 NAA and 0.3 mg·l-1 kinetin are transferred onto medium without hormones or with either hormone added separately, the β-1,3-glucanase content expressed per mg soluble protein increases approx. ten fold over a 7-d period. Under these inductive conditions, up to approx. 5% of the soluble protein is β-1,3-glucanase. The induction is inhibited by 〉90% when tissues are cultured over the same period on medium containing both hormones. This β-1,3-glucanase is developmentally regulated in the intact plant. It is a major component of the soluble protien in the lower leaves and roots but is not detectable in leaves near the top of the plant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00391416
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