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  • 1
    Electronic Resource
    Electronic Resource
    The effect of ammonium ions on uptake of glutamine and other amino compounds by cultured cells of rapeseed (1978)
    Springer
    Planta 139 (1978), S. 193-197 
    Details
    ISSN: 1432-2048
    Keywords: Ammonium ; Brassica ; Cell culture ; Glutamine ; Nitrogen nutrition and uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogen starvation for 24 h, particularly NH 4 + deprivation, of cultured repeseed (Brassica napus L.) cells caused them to develop the potential for a rapid increase in the rate of uptake of several amino compounds. Time-course studies, primarily using [14C]glutamine, showed that the increase in uptake rate continued for about 10 h after the 24-h N-starvation period. Addition of 2 mM NH 4 + to N-starved cells caused the increase in glutamin-uptake rate to cease, and the rate began to fall after 3–6 h to the level observed in cells grown continuously in the presence of NH 4 + . Neither pH changes in the medium caused by the presence of NH 4 + nor the competitive inhibition by NH 4 + of glutamine transport into the cells were responsible for the changes in the glutamine uptake rate. Similar results were obtained in time-course studies with [14]leucine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388629
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The isolation of auxotrophs from Datura innoxia Mill. cell cultures following recovery of arsenate-treated cells on feeder plates (1984)
    Springer
    Planta 160 (1984), S. 168-173 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture ; Datura ; Mutant (counterselection) ; Nitrate reductase (deficient mutant) ; Threonine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wild-type (Ph1) and adenine-requiring (Ad1) cell lines of Datura innoxia Mill. were used in experiments to evaluate arsenate as a growth-lethal compound and its use as a counterselection agent. These experiments led to the devising of methods for the recovery of Ph1 and Ad1 cells after arsenate treatment when plated at low density on feeder plates. The modified counterselection technique was then used to isolate three new auxotrophs from mutagenized suspensions of Ph1 cells, two of which were partially characterized. One, C18, requires casein hydrolysate for growth and lacks an active nitrate reductase; the other, JM3, will grow only when the medium contains threonine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392866
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill (1980)
    Springer
    Planta 149 (1980), S. 480-484 
    Details
    ISSN: 1432-2048
    Keywords: Adenine ; Auxotroph ; Cell cultures, variant lines ; Datura ; Pantothenate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A growth analysis of several presumptive “leaky” auxotrophs from Datura innoxia suspension cultures led to the discovery of an adenine-requiring cultures led to the discovery of an adenine-requiring cell strain (Ad1). Both Ad1 and Pn1, a pantothenate-requiring strain isolated earlier from these cultures, still require either adenine or pantothenate for growth after more than one year in culture. Attempts to select prototrophic revertants have failed. Ad 1 also grew well in a medium containing either 5-aminoimidazole-4-carboxamide ribotide or inosine instead of adenine; Pn 1 with pantoic acid alone but not at all in the presence of β-alanine or α-ketoisovalerate alone instead of pantothenate. Pn 1 cells starved of pantothenate for up to 4d and Ad1 of adenine for 10d or more resumed growth when transferred to appropriately supplemented media. Wild-type Datura cells grown on unsupplemented medium would not crossfeed the required nutrients to the auxotrophs. The starvation and cross-feeding experiment showed that both auxotrophs could be used in reconstruction experiments to develop enrichment-selection techniques for the isolation of more auxotrophs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385752
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    Paper (German National Licenses)
    Fulltext
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