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  • 1
    Electronic Resource
    Electronic Resource
    Selection and characterization of ura5 mutants of Histoplasma capsulatum (1988)
    Springer
    Molecular genetics and genomics 214 (1988), S. 348-352 
    Details
    ISSN: 1617-4623
    Keywords: Histoplasma capsulatum ; Fungus ; Orotidine-5′-monophosphate pyrophosphorylase ; Uracil ; Auxotroph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The combined use of non-aggregating Histoplasma capsulatum strains and a defined medium which allows quantitative plating of the yeast phase has allowed us to select 5-fluoroorotic acid (5-FOA)-resistant mutants of this dimorphic fungus. Approximately two-thirds of the 5-FOA-resistant strains were auxotrophic for uracil; all were deficient in orotidine-5′-monophosphate pyrophosphorylase (OMPpase) activity. One class of OMPpase mutant (α), which retained a low level of OMPpase activity, was auxotrophic in the yeast phase (37°C) but grew slowly in the mycelial phase (25°C) without exogenous uracil. This phenotype was not due to a temperature-sensitive OMPpase activity. Both wild-type and α mutants had a higher OMPpase activity in the mycelial phase than the yeast phase; this increased activity may be sufficient to allow mycelial growth of α mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337734
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a genetic transformation system for Histoplasma capsulatum: Complementation of uracil auxotrophy (1990)
    Springer
    Molecular genetics and genomics 221 (1990), S. 358-362 
    Details
    ISSN: 1617-4623
    Keywords: Histoplasma capsulatum ; Fungus ; Transformation ; Uracil ; Auxotroph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a simple and efficient transformation system for the dimorphic fungus Histoplasma capsulatum. Mutants of H. capsulatum defective in orotidine-5′-monophosphate pyrophosphorylase were transformed to prototrophy by the cloned URA5 gene of the filamentous fungus Podospora anserina. Abortive and mitotically stable transformants were obtained. The stable transformants had integrated copies of the plasmid, some in tandem head-to-tail orientation. Free plasmid identical to the transforming plasmid was present in some of the transformants. We obtained a transformation efficiency of up to 30 transformants/μg DNA for plasmid pPAura5-1 (9.2 kb). pPW2001, a smaller plasmid (4.7 kb) derived from pPAura5-1, transformed H. capsulatum more efficiently (up to 155 transformants/gm DNA).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259400
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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