ISSN:
1432-2048
Keywords:
Actin
;
Avena
;
Chlorophyll-a/b-binding protein
;
mRNA half-life
;
Phytochrome
;
β-Tubulin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have used a cell-free polysome-based in-vitro mRNA-degradation system to investigate the halflives of plant cell mRNAs. In order to establish the fidelity of the in-vitro system, we used cordycepin to determine the in-vivo half-lives of β-tubulin and actin mRNAs in the primary leaves of 4-d-old etiolated oat (Avena sativa L.) seedlings. The in-vitro rank order of half-lives for phytochrome A (45 min), β-tubulin (105 min), and actin (220 min) mRNAs mimicked the in-vivo rank order. A pulse of red light given to excised etiolated primary leaves caused an in-vivo reduction in the half-life of β-tubulin mRNA. The selectivity of the polysome-based system was further demonstrated by the decrease in the half-life of β-tubulin mRNA (from 105 min to 60 min) induced by a pulse of red light given to the etiolated oat seedlings prior to isolation of polysomes. Red light did not affect the apparent half-lives of phytochrome A or actin mRNAs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00195084
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