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  • 1
    ISSN: 1615-6102
    Keywords: Immunogold labeling ; Iron-superoxide dismutase ; Cyanobiont ; Azolla filiculoides ; Heterocysts ; Nitrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immunogold labeling and transmission electron microscopy were used to localize iron-superoxide dismutase (Fe-SOD) in the different cells of nitrogen-fixing cyanobacterial symbiont present within different leaf cavity groups ofAzolla filiculoides Lam. As evidenced by Western blotting and immunoprecipitation, Fe-SOD antibody fromAnabaena cylindrica recognized Fe-SOD in extracts of the cyanobiont and showed the same electrophoretic mobility and pattern as purifiedA. cylindrica Fe-SOD. In vegetative cells of the cyanobiont, Fe-SOD was mainly localized in the thylakoidal membranes and in the outer membrane. The labeling pattern was similar in vegetative cells of the various groups of leaf cavities examined except at the apex where a lower gold particle density was seen. In heterocysts of the leaf cavity groups containing high nitrogenase activity, Fe-SOD labeling was most pronounced and more intense than in vegetative cells. The Fe-SOD label was preferentially located throughout the heterocyst cytoplasm and in the honeycomb regions. In accordance with the decline in nitrogenase activity, the Fe-SOD gold particle density decreased significantly in heterocysts of basal leaf cavity group. The presence of Fe-SOD in regions of high nitrogenase protein levels, and the fact that the pattern of Fe-SOD label parallels that of nitrogenase activity support a role of Fe-SOD in the protection of nitrogenase against superoxide radicals.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 52 (1979), S. 99-107 
    ISSN: 1573-5036
    Keywords: Blue-green algae ; Biofertilisers ; Growth substances
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Biologically active compounds may be liberated from blue-green algae growing on the surface of moist soils. Such compounds may also be released as exudates from algae grown in liquid culture. This report describes inoculation of soils in pots, containing radish or tomato plants, with algal suspensions or exudates, which resulted in increased growth rates of both plants and increased their overall yield. Autoclaved exudates were generally as effective as fresh exudates. Interaction of effects between the various active substances depends on the algal species and method by which the soils are amended.
    Type of Medium: Electronic Resource
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