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  • 1
    Electronic Resource
    Electronic Resource
    Structure and evolution of supernumerary chromosomes in the Pacific giant salamander, Dicamptodon tenebrosus (2000)
    Springer
    Chromosome research 8 (2000), S. 477-485 
    Details
    ISSN: 1573-6849
    Keywords: B chromosome ; Dicamptodon tenebrosus ; genome evolution ; repetitive DNA ; salamander ; supernumerary chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the genetic make-up and plausible origins of the supernumerary (B) chromosomes of the Pacific giant salamander, Dicamptodon tenebrosus, from the Pacific Northwest of North America. These salamanders have variable numbers of B chromosomes, from 0 to 10 per individual. Salamanders from the most southerly and northerly regions of the species' range have lower average numbers of B chromosomes than salamanders in the middle of the range. To assess how the supernumerary chromosomes originated in D. tenebrosus, B chromosome DNA was isolated by microdissection and amplified by degenerate oligonucleotide-primed PCR. The B chromosome DNA hybridized similarly to genomic DNA from individuals of D. tenebrosus and the related species D. copei and D. ensatus, thus demonstrating that the supernumerary chromosomes were derived from the normal chromosome complement. Unique hybridization bands in both D. copei and D. tenebrosus suggest that the shared sequences have evolved independently.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009215621509
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Differences of histone H4 acetylation and replication timing between A and B chromosomes of Brachycome dichromosomatica (1997)
    Springer
    Chromosome research 5 (1997), S. 233-237 
    Details
    ISSN: 1573-6849
    Keywords: acetylated histone H4 ; B chromosome ; heterochromatin ; plants ; replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differences are demonstrated between A (transcriptionally active) and B (transcriptionally inactive) chromosomes that are characterized by a different level of histone H4 acetylation and a different timing of DNA replication. These differences between the chromatin of A and B chromosomes were found after immunolabelling of chromsomes of Brachycome dichromosomatica with antibodies specific for different acetylated forms (lysine 5, 8, 12 and 16) of histone H4. In contrast to the A chromosomes, which are labelled brightly in their entirety, the transcriptionally inactive B chromosomes are faintly labelled with antibodies against H4Ac5 and H4Ac8. No such difference between the chromosomes is found after immunostaining with the other antibodies H4Ac12 and H4Ac16. Analysis of DNA replication timing in root-tip meristems suggests that B chromosomes are labelled late in S-phase compared with A chromosomes. After C-banding the B chromosome appeared to have a similar amount of heterochromatin to the A chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:CHRO.0000032297.10876.86
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A monophyletic origin of the B chromosomes ofBrachycome dichromosomatica (Asteraceae) (1999)
    Springer
    Plant systematics and evolution 219 (1999), S. 127-135 
    Details
    ISSN: 1615-6110
    Keywords: Asteraceae ; Brachycome ; B chromosome ; polymorphism ; evolution ; karyotyping ; centromere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The A and B chromosomes of different karyotype variants (cytodemes A1, A2, A3 and A4) ofBrachycome dichromosomatica were analysed by computer-aided chromosome image analysis and fluorescencein situ hybridisation (FISH). Ribosomal DNA and the B chromosome-specific sequence Bd49 were detected on all B chromosomes. In addition to minor size variation of the Bs, polymorphism of the rDNA and Bd49 position and copy number revealed two major types of B chromosomes. The B chromosomes of all the cytodemes were indistinguishable from each other in length, but that of A3 showed evidence of rearrangements consistent with its long-term geographic isolation. The results presented suggest a monophyletic origin of the B chromosomes ofB. dichromosomatica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01090304
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    Paper (German National Licenses)
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