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  • Life and Medical Sciences  (8)
  • B chromosomes  (5)
  • Secale cereale  (5)
  • tomato  (5)
  • Cell culture  (3)
  • 1
    Electronic Resource
    Electronic Resource
    In vitro culture of human prostatic tissue (1977)
    Springer
    Urological research 5 (1977), S. 207-210 
    Details
    ISSN: 1434-0879
    Keywords: Cell culture ; Prostate ; Collagenase ; Epithelial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A procedure is described which yields a significant percentage of long-term mixed cell cultures of human prostatic tissue. Attempts were made to suppress the proliferation of stromal fibroblasts and to characterize the cultured cells as those of prostatic origin. The problems associated with establishing epithelial cell lines are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263737
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  • 2
    Electronic Resource
    Electronic Resource
    Nitrate reductase activity in Paul's scarlet rose suspension cultures and the differential role of nitrate and molybdenum in induction (1978)
    Springer
    Planta 141 (1978), S. 183-189 
    Details
    ISSN: 1432-2048
    Keywords: Antimetabolites ; Cell culture ; Enzyme induction ; Molybdenum ; Nitrate reductase ; Rosa, Paul's Scarlet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction of nitrate reductase (EC 1.6.6.1) activity was measured in Paul's Scarlet rose cell suspensions cultured in media containing nitrate (NO 3 - ) or urea (U) as nitrogen source, and with (+Mo) or without molybdenum (-Mo). There was a lag of 30 min during induction by NO 3 - in +Mo cultures but no lag occurred during induction after adding Mo to NO 3 - -Mo or to U-Mo cultures preincubated with NO 3 - . Actinomycin D, cycloheximide, and puromycin completely blocked induction by NO 3 - , but had no effect on the initial rate of induction by Mo. Cycloheximide and puromycin blocked induction by NO 3 - more quickly than actinomycin D. Induction by NO 3 - appeared to involve mRNA-dependent synthesis of apoprotein followed by rapid activation with molybdenum in intact cells independently of protein synthesis. Nitrate-induced apoprotein appeared less stable than the holoenzyme. When induced by NO 3 - in the absence of Mo, apoprotein concentration was about half the amount of maximally induced nitrate reductase. Cycloheximide stabilised preformed nitrate reductase which disappeared steadily in the presence of puromycin. Apoprotein was not stabilised by either antimetabolite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387887
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrate reductase activity and growth in Paul's Scarlet rose suspension cultures in relation to nitrogen source and molybdenum (1976)
    Springer
    Planta 133 (1976), S. 27-34 
    Details
    ISSN: 1432-2048
    Keywords: Rose ; Paul's Scarlet ; Cell culture ; Nitrate reductase ; Molybdenum ; Enzyme induction ; Enzyme assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and nitrate reductase activity were measured in Paul's Scarlet rose cell suspensions, cultured in media purified from molybdenum and containing nitrate or urea as sole nitrogen source with or without added Mo. Urea could replace nitrate to yield 80% of the fresh weight in nitrate medium. Nitrate reductase activities were compared by in vivo and in vitro assays. The latter varied due to inactivation during extraction. Compared with activities in cells in complete NO3 - medium, activity in NO3 --Mo cells was reduced to 30% and, in urea-grown cells, to trace amounts. Increases in nitrate reductase activity were found when NO3 - alone was added to NO3 - or urea+Mo cultures. In NO3 --Mo cultures, Mo alone or with NO3 - caused a similar increase in activity, whereas urea-Mo cultures required both NO3 - and Mo for enzyme induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386002
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  • 4
    Electronic Resource
    Electronic Resource
    The influence of B chromosomes on rDNA organization in rye interphase nuclei (1995)
    Springer
    Chromosome research 3 (1995), S. 487-491 
    Details
    ISSN: 1573-6849
    Keywords: B chromosomes ; interphase chromatin ; rDNA organization ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Patterns of rye rDNA organization in interphase nuclei were studied through the use ofin situ hybridization in spreads of root meristem cells from plants with and without B chromosomes (Bs). In cells from plants without Bs each rDNA locus is organized as a single perinucleolar knob of condensed chromatin with decondensed chromatin inside the nucleolus. In plants with Bs there is a marked modification of the pattern, found in more than 23% of nuclei, which involves several regions of condensed chromatin interspersed with decondensed chromatin inside the nucleolus. This B-induced alteration in rDNA interphase organization suggests a change in expression of the rRNA genes located on the A chromosomes probably related to the reduction in nuclear RNA observed previously in plants with Bs. The influence of the Bs on the expression of A chromosome genes, through rearrangement of interphase chromatin, could provide the basis of an explanation for some of the known phenotypic effects of B chromosomes in rye.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00713963
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH) (1995)
    Springer
    Chromosome research 3 (1995), S. 466-472 
    Details
    ISSN: 1573-6849
    Keywords: B chromosome ; DNA sequence composition ; fluorescencein situ hybridization ; repetitive sequence ; Secale cereale ; translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A–B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00713960
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  • 6
    Electronic Resource
    Electronic Resource
    Painting rye B chromosomes in wheat: interphase chromatin organization, nuclear disposition and association in plants with two, three or four Bs (1996)
    Springer
    Chromosome research 4 (1996), S. 195-200 
    Details
    ISSN: 1573-6849
    Keywords: B chromosomes ; genomicin situ hybridization ; nuclear architecture ; Secale cereale ; Triticum aestivum cv ; Lindström
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The B chromosomes (Bs) of rye (Secale cereale) have been studied at interphase in terms of their chromatin organization, patterns of nuclear disposition and physical association in plants with two, three, and four Bs. The study was made in the Lindström strain of hexaploid wheat, which carries the rye Bs as an addition line, byin situ hybridization with a B-specific probe and by genomicin situ hybridization (GISH) with rye genomic DNA, enabling whole chromosome painting. Repetive sequences common to the As and Bs of rye allow for visualization of the rye B at interphase in the wheat background. A B-specific probe enables the orientation of two or more Bs to be determined, and the combination of both probes used together gives information on the disposition of the Bs and on their patterns of physical association within the nucleus. The Bs form linear ‘strings’, and the ends of their long arms, which can be detected by the B-specific probe, are usually located within the hemisphere of the nucleus that has the least condensed chromatin. There is dose-dependent association, and even numbers (2B, 4B) have a greater preference for association than odd ones (3B).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02254959
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  • 7
    Electronic Resource
    Electronic Resource
    High salt tolerance potential in Lycopersicon species during germination (1986)
    Springer
    Euphytica 35 (1986), S. 575-582 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; salinity ; germination ; germplasm ; breeding ; salt-tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The potential to improve seed germination responses to salinity was evaluated for 13 accessions representing six wild Lycopersicon species and 20 accessions of L. esculentum. Germination response times increased in all accessions at 100 mM NaCl. Analysis indicated that one accession of L. peruvianum (PI126435) germinated faster under high salinity than all other accessions and was closely followed by L. pennellii (LA716). The fastest germinating L. esculentum accession, PI174263, ranked third. Additional wild ecotypes exhibiting rapid germination at 100 mM NaCl were identified among L. pimpinellifolium and L. peruvianum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021866
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  • 8
    Electronic Resource
    Electronic Resource
    A greenhouse screening method forPyrenochaeta lycopersici resistance in tomatoes (1989)
    Springer
    Euphytica 40 (1989), S. 187-191 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; Pyrenochaeta lycopersici ; corky root ; brown root rot ; resistance ; greenhouse screening method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse screening method for corky root (Pyrenochaeta lycopersici) resistance in the tomato (Lycopersicon esculentum) is described in detail. In determining the reliability of the method, known resistance sources within wild asccessions, commerical cultivars and breeding lines were evaluated for resistance in fields naturally infested with corky root and their response compared under greenhouse conditions in soil heavily infested with corky root and artifically maintained at temperatures between 10–13°C. The procedure developed can be used year round and improves the efficiency of recovering resistant progeny in large segregrating populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024510
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  • 9
    Electronic Resource
    Electronic Resource
    Generation means analysis of right-censored response-time traits: Low temperature seed germination in tomato (1990)
    Springer
    Euphytica 48 (1990), S. 239-244 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; cold tolerance ; seed germination ; genetic analysis ; missing data ; response-time data
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In studies to determine the inheritance of response-time traits, such as time to seed germination, some viable individuals may fail to respond during an experiment. If these right-censored observations are ignored, sample means and variances will be underestimated. This is illustrated using data from time to seed germination at 9°C for Lycopersicon esculentum (Mill.) fast germinating PI 120256, slow-germinating T3 and their reciprocal F1, F2 and backcross progeny. This paper presents methods to detect and to accommodate right-censored data in generation means analysis. Genetic interpretations derived from corrected and uncorrected estimates of generation means and variances are compared. Correction for right-censoring increased estimates of environmental and phenotypic variances, and decreased heritability estimates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023656
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  • 10
    Electronic Resource
    Electronic Resource
    Improvement of tomato flavor by genetically increasing sugar and acid contents (1983)
    Springer
    Euphytica 32 (1983), S. 845-855 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; flavor ; sensory evaluation ; soluble solids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Advanced high sugar and acid breeding lines of tomatoes (Lycopersion esculentum Mill.) were rated higher in sweetness, sourness and overall flavor intensity than the standard cultivars Cal Ace or T3. Titratable acidity and soluble solids content were major contributors to differences in overall flavor intensity. The results demonstrate that significant improvement in tomato flavor can be attained by increasing sugar and acid contents in tomato fruits by genetic manipulation. Current evidence indicates that breeding for high soluble solids in horticulturally acceptable tomato cultivars is justified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042166
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