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  • 1
    Electronic Resource
    Electronic Resource
    Male sterility caused by cytoplasm of Brassica oxyrrhina in B. campestris and B. juncea (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 285-287 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility ; Brassica oxyrrhina ; B. campestris ; B. juncea ; Alien gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Synthetic alloploid Brassica oxyrrhina (2n = 18, OO) x B. campestris (2n = 20, AA) was repeatedly backcrossed with B. campestris to place B. campestris nucleus in the cytoplasm of B. oxyrrhina. Alloplasmic plants, obtained in BC5 generation, were stably male sterile but mildly chlorotic during initial development. Synthetic alloploid B. oxyrrhina-campestris was also hybridized with B. juncea to transfer B. oxyrrhina cytoplasm. Segregation for green and chlorotic plants was observed in BC1 and BC2 generations. By selection, however, normal green male sterile B. juncea was obtained in BC3. Pollen abortion in both B. campestris and B. juncea is post-meiotic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225965
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chloroplast substitution overcomes leaf chlorosis in a Moricandia arvensis-based cytoplasmic male sterile Brassica juncea (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1179-1182 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica juncea ; Moricandia arvensis ; Cytoplasmic male sterility ; Chlorosis correction ; Cytoplasmic hybrids ; Chloroplast substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A male sterile Brassica juncea line based on Moricandia arvensis cytoplasm was developed previously by backcrossing the somatic hybrid M. arvensis+B. juncea, and the gene for restoring fertility was introgressed. The CMS line is very severely chlorotic because of the presence of alien chloroplasts and flowering is delayed by 30–40 days, making it unsuitable for the exploitation of heterosis. We have resorted to another cycle of protoplast fusion between green fertile B. juncea and chlorotic male sterile B. juncea, and developed green male-sterile plants. Molecular analysis revealed that in green male-sterile plants chloroplasts of M. arvensis origin were substituted by those from B. juncea, giving rise to intergeneric cytoplasmic hybrids with mitochondria of M. arvensis origin. With the development of dark-green male-sterile plants, the CMS fertility restoration system is suitable for the production of hybrid mustard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051007
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 517-521 
    Details
    ISSN: 1432-2242
    Keywords: Ogu cytoplasmic male sterility ; Brassica juncea ; Chlorosis correction ; Protoplast fusion ; Mitochondrial recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male sterility conferred by ogu cytoplasm of Raphanus sativus has been transferred to Brassica juncea cv ‘RLM 198’ from male-sterile B. napus through repeated backcrossing and selection. The male-sterile B. juncea is, however, highly chlorotic and late. It has low female (seed) fertility and small contorted pods. To rectify these defects, protoplasts of the male sterile were fused with normal ‘RLM 198’ (green, self fertile). Four dark green, completely male-sterile plants were obtained and identified as putative cybrids. All the plants were backcrossed three times with ‘RLM 198’. Mitochondrial and chloroplast DNA analysis of backcross progeny confirmed hybridity of the cytoplasm. The restriction pattern of the chloroplast DNA of progeny plants of three cybrids (Og 1, Og 2, Og 3) was similar to that of the green self-fertile ‘RLM 198’ and indicated that the correction of chlorosis resulted from chloroplast substitution. The chloroplast DNA of the lone progeny plant of the fourth cybrid (Og 10) could not be analyzed because the plant was stunted and had only a few leaves. When total cellular DNA was probed with mitochondrial probes coxI and atpA it was found that the cybrids had recombinant mitochondria. The chlorosis-corrected plants were early flowering and had vastly improved seed fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222982
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Intergeneric hybridization between Diplotaxis siifolia, a wild species and crop brassicas (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 537-541 
    Details
    ISSN: 1432-2242
    Keywords: Brassica campestris ; B. juncea ; B. napus ; Diplotaxis siifolia ; Intergeneric hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Attempts were made to obtain intergeneric hybrids between Diplotaxis siifolia, a wild species, and cultivars of Brassica (B. campestris, B. juncea, and B. napus). The crosses showed unilateral incompatibility. When the wild species was used as female parent, pollen germination and pollen tube growth were normal, but hybrid seeds aborted due to post-fertilization barriers. Reciprocal crosses (cultivars as female parent) showed strong pre-fertilization barriers; although pollen grains showed germination, pollen tubes failed to enter the stigma. Hybrids were realized in two of the crosses, D. siifolia x B. juncea and D. siifolia x B. napus, through ovary culture. The hybrids were multiplied in vitro by multiplication of axillary shoots, or somatic embryogenesis. Detailed studies were carried out on the hybrid D. siifolia x B. juncea. F1 hybrids had shrivelled anthers and were pollen sterile. Amphiploids of this hybrid showed 60% pollen fertility and produced seeds upon self-pollination as well as backcross pollination with the pollen of B. juncea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226756
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    Paper (German National Licenses)
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