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  • 1
    Electronic Resource
    Electronic Resource
    Fine physical mapping of the rice stripe resistance gene locus, Stvb-i (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 59-63 
    Details
    ISSN: 1432-2242
    Keywords: Keywords Rice ; Stripe disease resistance gene ; Stvb-i ; BAC ; Physical map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Stvb-i gene confers stripe disease resistance to rice. For positional cloning, we constructed a physical map spanning 1.8-cM distance between flanking markers, consisting of 18 bacterial artificial chromosome (BAC) clones, around the Stvb-i locus on rice chromosome 11. The 18 clones were isolated by screening a BAC library derived from a japonica cultivar, Shimokita, with three Stvb-i-linked RFLP markers and DraI-digested DNAs of a yeast artificial chromosome (YAC) clone. The results of Southern hybridization and restriction enzyme analyses indicated that these BAC clones are contiguous and cover about a 700-kb region containing the Stvb-i allele. Utilizing end and internal fragments of the BAC insert DNAs, 33 molecular markers were generated within a small chromosomal region including the Stvb-i locus. Genotyping analysis with these markers for a resistant cultivar and four nearby recombinants selected from 120 F2 individuals indicated that Stvb-i is contained within an approximately 286-kb region covered with two overlapping BAC clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051449
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of peritoneal insufflation on hepatic and renal blood flow (1994)
    Springer
    Surgical endoscopy and other interventional techniques 8 (1994), S. 759-761 
    Details
    ISSN: 1432-2218
    Keywords: Laparoscopic surgery ; Peritoneal insufflation ; Organ blood flow ; Liver ; Kidney ; Carbon dioxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of peritoneal insufflation with carbon dioxide on hepatic and renal blood flow have not been reported hitherto. We evaluated these effects in a porcine model of abdominal laparoscopic surgery. Seven anesthetized pigs underwent peritoneal insufflation in a step-wise manner to create intraabdominal pressures of 6, 12, 18 and 24 mmHg, and changes in the arterial and venous pressure, arterial blood gases, and hepatic and renal blood flow were monitored. Both the hepatic and renal blood flow decreased as the intraabdominal pressure increased. Therefore, in order to carry out laparoscopic abdominal surgery safely in patients with hepatic or renal impairment, low intraabdominal pressures or noninsufflating techniques are recommended.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00593435
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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