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  • Phaseolus  (3)
  • BIM 23014  (1)
  • BPC 157  (1)
  • 1
    ISSN: 1572-9818
    Schlagwort(e): CFLP ; Cleavase I ; Phaseolus ; phylogeny ; ptDNA intergenic regions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The CFLP methodology was applied for Cleavase I site detection within ptDNA intergenic regions (atpB-rbcL and rps14-psaB) at both interspecific and intraspecific levels in the genus Phaseolus. Optimal Cleavase I reaction temperature was 55 °C and the semi-dry electrophoretic transfer was more efficient than the original capillary one. Cleavase reactions yield a high number of fragments as compared to PCR-RFLP and allowed differentiation within and between landraces and wild forms of the Lima bean (Phaseolus lunatus L.) originating from Andean and Mesoamerican regions of Latin America. From sequencing data and using stemloop program (GCG, Madison), congruent numbers of hairpins/fragments were identified within the sequences, highlighting the robustness of the Cleavase I. Our results pointed out the ubiquity of short conserved motifs amongst a geographically localized group of species. In the vicinity of these motifs, synapomorphic-like substitutions were frequently observed. A phylogenetic tree based on these sequences is congruent with the CFLP pattern as well as with the widely accepted phylogeny of the genus. The usefulness of this new tool as alternative and/or complementary to PCR-RFLP technology on ptDNA is suggested and discussed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1432-1041
    Schlagwort(e): BIM 23014 ; Somatostatin analogue ; growth hormone ; GHRH test ; OGTT ; adverse effects
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary We have studied the pharmacokinetics and the effects of BIM 23014 (BIM), a new, long-acting octapeptide somatostatin analogue, on basal and stimulated GH secretion in normal men. BIM 250 μg sc significantly reduced a GHRH-induced increase in plasma GH. The continuous sc administration of BIM for 24 h dramatically blunted spontaneous GH secretion; 2000 and 3000 μg daily reduced GH secretion to a greater extent than 1000 μg daily. During these experiments a significant negative correlation (r−0.66) was found between plasma GH and BIM levels. Acute sc administration of 1000 μg BIM significantly reduced the rise in plasma GH observed in the second part of the oral glucose tolerance test. Plasma BIM levels peaked around 30 min, and the elimination half life was 90 min. Plasma BIM levels were below 1 ng/ml 6 h after the injection of 1000 μg BIM, and at that time GH started to rise again. We conclude that BIM 23014 250 to 1000 μg sc is able to reduce the plasma GH response to GHRH or to the fall in glucose following an oral glucose tolerance test; a constant infusion of BIM, in doses 1000 μg daily, dramatically suppresses spontaneous GH secretion; 2000 μg/day by chronic subcutaneous infusion was the most effective dose of BIM in the suppression of GH secretion, and was associated only with minor adverse effects.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 218 (1999), S. 43-54 
    ISSN: 1615-6110
    Schlagwort(e): Fabaceae ; Phaseolus ; Phaseolus lunatus ; Taxonomy ; germplasm ; evolution ; origin ; allozyme ; Latin America
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An investigation was made of the phylogenetic relationships among wild accessions of Lima bean (Phaseolus lunatus) and wild allies of Mesoamerican and Andean origins, using electrophoresis of seed storage proteins and isozymes. Mesoamerican wild species are phylogenetically more distant fromP. lunatus than Andean species, and apparently belong to the tertiary gene pool of Lima bean. The Andean wild species, which are investigated for the first time, reveal a high similarity to the Lima bean, and particularly with its Mesoamerican gene pool. These Andean species probably constitute a secondary gene pool of Lima bean, and are thus of considerable interest in the context of genetic improvement of the crop. Based on these observations, an Andean origin is suggested for the Andean wild species and forP. lunatus. These results point out the importance of collecting and conserving AndeanPhaseolus germplasm.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    European archives of oto-rhino-laryngology and head & neck 254 (1997), S. S9 
    ISSN: 1434-4726
    Schlagwort(e): Capsaicin ; Rhinitis ; BPC 157 ; Mastocytes ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Protection of BPC 157 on capsaicin-induced rhinitis was studied in Wistar rats for its effect on mastocyte infiltration, degranulation and inflammatory cell infiltration. Animals were pretreated with 10 μg/kg, 10 ng/kg or 2 ml saline i.p. and capsaicin (0.05 ml/nostril of 1750 nmol/l sol.) was applied intranasally. They were then euthanized at 1, 3 and 12 h after capsaicin provocation. Nasal mucosa was analyzed and scored for mastocyte infiltration, degranulation and inflammatory cell infiltration. BPC 157 pretreatment significantly prevented mastocyte infiltration at 1 h. Polymorphonuclear leukocyte infiltration was significantly reduced in rats pretreated with 10 μg/kg BPC 157. A dose-dependent effect of BPC 157 pretreatment was demonstrated only for polymorphonuclear leukocyte infiltration at 12 h.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 96 (1997), S. 115-123 
    ISSN: 1573-5060
    Schlagwort(e): beans ; breeding ; intercropping ; multiple cropping ; Phaseolus ; relay cropping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Common bean, Phaseolus vulgaris L. is intercropped or relay cropped with maize in many Andean highlands of Colombia and Peru. Breeding beans for the target multiple cropping systems is essential for the development of productive and sustainable agriculture for the Andean smallholders. Outline of the breeding programme should follow the farming system approach with the establishment of on-farm trials and early farmers involvement. Bean breeding is oriented to minimize intercrop competition and to stabilize complementarity with maize. Genetic traits needed for improved varieties are divided as follows : traits not interacting with the cropping systems, traits specific to intercrops and traits related with socioeconomic and seed quality aspects. Screening, prebreeding and recombination nursery are better made under sole cropping while varietal improvement and on-farm trials are conducted under the target multiple cropping systems. Breeding schemes may involve recurrent, pedigree and bulk hybrid selection. The given application concerns the genetic improvement of P. coccineus, P. polyanthus and interspecific hybrids of P. vulgaris for both simultaneous and relay intercropping in Colombia and Peru. Earliness, cold tolerance, resistance to fungus diseases (mainly Ascochyta leaf blight and anthracnosis) and seed yield potential were the major objectives of the bean improvement programme. Priority has been given to the exploitation of the large diversity available in the secondary gene pool of common bean.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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