ISSN:
1432-072X
Keywords:
Branched-chain fatty acids
;
Fatty acid synthase
;
Thiolactomycin
;
Selective inhibitor
;
Bacillus subtilis
;
Bacillus cereus
;
Bacillus insolitus
;
Escherichia coli
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The antibiotic, thiolactomycin, is known to selectively inhibit the Type II straight-chain fatty acid synthase (monofunctional enzyme system, e.g. Escherichia coli enzyme) but not Type I straight-chain fatty acid synthase (multifunctional enzyme system, e.g. Saccharomyces cerevisiae enzyme). We have studied the effect of thiolactomycin on the branched-chain fatty acid synthases from Bacillus subtilis, Bacillus cereus, and Bacillus insolitus. Fatty acid synthase from all three Bacilli was not inhibited or only slightly inhibited by thiolactomycin. E. coli synthase, as expected, was strongly inhibited by thiolactomycin. Branched-chain fatty acid synthase from Bacillus species is a monofunctional enzyme system but, unlike Type II E. coli synthase, it is largely insensitive to thiolactomycin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00288719
Permalink
