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Identification of T4 gene 25 product, a component of the tail baseplate, as a 15K lysozyme (1986)

Szewczyk, Boguslaw ; Bienkowska-Szewczyk, Krystyna ; Kozloff, Lloyd M.

Springer

Molecular genetics and genomics 202 (1986), S. 363-367

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ISSN: 1617-4623

Keywords: Bacteriophage T4 ; Gene 25 product ; Tail baseplate ; Lysozyme

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary The proteins synthesized in Escherichia coli B cells after infection with various T4 bacteriophage tail baseplate mutants were analysed by the immunoblotting method for the presence of the 15 Kilodalton lysozyme found in phage T4 particles. Using three different antisera: anti-phage, anti-baseplate and anti-15K lysozyme, it has been found that the 15K lysozyme is not present in lysates of bacteria infected with T4 gene 25 amber mutants. The 15K lysozyme was also found to be expressed in E. coli B cells transformed with a plasmid containing only a small portion of the T4 genome but which included T4 gene 25. These observations indicate that the 15K lysozyme is the gene 25 product.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00333263

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Automatic matrix determination in four dye fluorescence-based DNA sequencing (1996)

Yin, Zhongbin ; Severin, Jessica ; Giddings, Michael C. ; [et al.]

Weinheim : Wiley-Blackwell

Electrophoresis 17 (1996), S. 1143-1150

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ISSN: 0173-0835

Keywords: DNA sequencing ; Matrix ; Algorithm ; Multicomponent analysis ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: The four dye fluorescence detection strategy is a widely used approach to automated DNA sequence analysis. An important aspect of data processing in this approach is the multicomponent analysis to deduce the concentrations of four fluorophores from fluorescence emission intensities at four different wave-lengths. This requires knowledge of the correct transformation matrix M. The matrix M is a function both of the fluorophores employed and the fluorescence detection system. M is typically determined either by a calibration process with individual dyes, or by choosing four well-separated individual peaks corresponding to the four different dyes. Both are time-consuming and complicated procedures for routine use. An automatic scheme for finding M directly from raw sequence data is presented here. This facilitates data analysis and the underlying algorithm may also find utility in other multispectral applications.

Additional Material: 6 Ill.