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  • 1
    Electronic Resource
    Electronic Resource
    FISH Mapping of Bovine U21, U1 and U7 Molecular Markers to River Buffalo Chromosomes 3p, 5q and 5p (1997)
    Springer
    Chromosome research 5 (1997), S. 337-340 
    Details
    ISSN: 1573-6849
    Keywords: cattle ; chromosome ; cosmid ; fluorescence in situ hybridization ; river buffalo ; synteny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three bovine cosmid-derived microsatellites (IDV-GA49, IDVGA7 and IDVGA47), previously assigned to cattle syntenic groups U1, U7 and U21, respectively, were fluorescence in situ hybridization (FISH) mapped to river buffalo (Bubalus bubalis, L., 2n= 50) chromosomes (BBU) 3p22 (IDVGA47, U21), BBU 5q21 (IDVGA49, U1) and BBU 5p19 (IDVGA7, U7) using sequential FISH and R-banding techniques. These localizations allowed the assignment, for the first time, of the bovine syntenic groups U21, U1 and U7 to specific river buffalo chromosomes. FISH mapping of IDVGA7 (U7) to cattle rob(1;29) p-arms confirms the banding homologies between BTA 29 and BBU 5p and further supports the idea that cattle standard karyotypes need adjustments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:CHRO.0000038765.65580.22
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  • 2
    Electronic Resource
    Electronic Resource
    Six bovine cosmid-derived microsatellites mapping different syntenic groups are fluorescence in situ hybridization mapped to six river buffalo chromosomes (1997)
    Springer
    Chromosome research 5 (1997), S. 541-543 
    Details
    ISSN: 1573-6849
    Keywords: cosmid ; fluorescence in situhybridization ; nomenclature ; R-banding ; river buffalo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Six bovine cosmid-derived microsatellites (IDVGA53, BTA3/U6; IDVGA61, U13; IDVGA41, BTA12/U27; IDVGA32, BTA15/U19; IDVGA59, BTA26/U26 and IDVGA71, U8), previously assigned to cattle chromosomes, were FISH-mapped to river buffalo chromosomes (BBU) 6q15, 8q34, 13q15, 16q25, 23q22 and 24q13 respectively. Sequential FISH/RBA-banding allowed the precise identification of chromosomes and localization of probe-signals on chromosome bands. These localizations allowed us to assign indirectly, for the first time, six bovine syntenic groups to river buffalo chromosomes, thereby extending its physical map. The localization of IDVGA71 (bovine U8) to the marker BBU24 adds further information to resolve definitively cattle chromosome ambiguities involving cattle chromosomes 25, 27 and 29.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018441703221
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A repeated chromosomal DNA sequence is amplified as a circular extrachromosomal molecule in rice (Oryza sativa L.) (1990)
    Springer
    Molecular genetics and genomics 222 (1990), S. 58-64 
    Details
    ISSN: 1617-4623
    Keywords: Bal31 ; DNA amplification ; Extrachromosomal DNA ; Pulsed field gel electrophoresis ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The plasmid pE10 is a pBR322-derived plasmid carrying a 4.5 kb rice (Oryza sativa L.) repeated DNA sequence. The cloned sequence has been shown to be amplified in cultured rice cells. The analysis of practically intact chromosomal rice DNA molecules by pulsed field gel electrophoresis has now shown that the amplification is associated with the appearance of extrachromosomal molecules. In fact, pE10 hybridizes exclusively with unfractionated DNA from leaf protoplasts, while it recognizes predominantly an extrachromosomal DNA molecule (ECD) of about 45 kb and its multiples in the case of protoplasts from cultured cells. Insensitivity to the action of the exonuclease Bal31 suggests that the molecule is circular. Analysis of restriction endonuclease products with both standard horizontal and pulsed field gel electrophoresis suggest that the extrachromosomal DNA, and its chromosomal counterpart, is composed of tandemly repeated units of about 7 kb. Thus, the smaller extrachromosomal circle should contain 6–7 repeats, while the sequence cloned in pE10 is a subset of this repeat. The extrachromosomal DNA represents about 1 % of total rice DNA and its level of amplification is not affected by the different phases of growth in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283023
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    Paper (German National Licenses)
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