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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 62 (1982), S. 53-58 
    ISSN: 1432-2242
    Keywords: Barley ; Interchange ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tuleen 346 barley is a triple homozygote for 3 unequal interchanges, T1-5v, T2-6y and T3-7d, which were induced independently in the variety, ‘Bonus’. It has great potential value in studying differences in behaviour and position among chromosomes within the same cell in barley and its hybrids since at least 5 and usually all 7 chromosome types can be identified in Feulgen preparations, compared with only 3 types in normal barley. Measurement of chromosome arm and satellite lengths in 10 haploid root-tip metaphases showed that in all comparisons except of the longest with the next longest, the 7 chromosome types were distinct in total length (P〈0.001–0.05) and in 8 of the 10 cells, the longest chromosome had a smaller arm ratio than the next longest. In these preparations, each of the 5 shortest chromosomes was easily identifiable by size and morphology. The longest chromosome was about 2.2 times as long as the shortest. C-banding showed that each of the 7 chromosome types had a clearly unique band pattern. The C-band patterns of interchange chromosomes confirmed and increased the precision of previously published breakpoint locations, viz. the short arms of chromosomes 1, 2, 3 and 7 and the long arms of chromosomes 5 and 6.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Genomic probe ; Blocking DNA ; Chemiluminescence ; Species identification ; Cereals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Labelled total genomic DNA was used as a probe in combination with blocking DNA to discriminate between taxonomically closely related species in the genera Hordeum and Secale. Discrimination was possible both by Southern hybridization to size-fractionated restriction enzyme digests of genomic DNA and by in situ hybridization to chromosome preparations. To distinguish between two species (e.g. H. vulgare and H. bulbosum), genomic DNA from one species was used as the labelled probe, while unlabelled DNA from the other species was applied at a much higher concentration as a block. The blocking DNA presumably hybridized to sequences in common between the block and the labelled probe, and between the block and DNA sequences on the membrane or chromosomes in situ. If so, mainly species-specific sequences would remain as sites for probe hybridization. These species-specific sequences are dispersed and represent a substantial proportion of the genome (unlike many cloned, species-specific sequences). Consequently, rapid nonradioactive methods detected probe hybridization sites satisfactorily. The method was able to confirm the parentage of hybrid plants. It has potentially wide application in plant breeding for the detection of alien DNA transfer, and it can be easily adapted to many species.
    Type of Medium: Electronic Resource
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