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  • 1
    Electronic Resource
    Electronic Resource
    Tests for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the Hordeum bulbosum system (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 509-513 
    Details
    ISSN: 1432-2242
    Keywords: Gametoclonal variation ; Barley doubled haploids ; Wheat doubled haploids ; Hordeum bulbosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate whether the Hordeum bulbosum system of doubled haploid production generates gametoclonal variation, populations of second generation doubled haploid lines were developed from first generation doubled haploid lines of two barley varieties and three wheat genotypes. In barley, no variation between doubled haploids from doubled haploids was detected for a range of quantitative characters, suggesting the absence of any gametoclonal effects. However, the original selfed-seed stocks were shown to contain cryptic allelic variation for some of the characters investigated. In wheat, gametoclonal variation was detected for ear emergence time, plant height and yield, and its components for two out of the three genotypes investigated. The type and range of variation was similar to that reported from studies of somaclonal variation from immature embryos and gametoclonal variation from anther culture. Generally, the effects appeared to reduce the yield performance of individual lines. The difference in response between the two species and the consequences for the use of the doubled haploid system in breeding programmes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276758
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  • 2
    Electronic Resource
    Electronic Resource
    Doubled haploid production in winter wheat and triticale genotypes, using the Hordeum bulbosum system (1986)
    Springer
    Euphytica 35 (1986), S. 1045-1051 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum bulbosum ; Triticum aestivum ; wheat ; triticale ; haploids ; doubled haploids ; crossability ; seed set ; embryo regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An attempt was made to produce doubled haploids on 16 winter wheat and six spring and winter triticale genotypes thought to carry genes for interspecific incompatibility. The potential for haploid production was maximized by the use of Hordeum bulbosum genotypes selected for high crossability on crossable wheat genotypes, the use of two post-pollination applications of gibberellic acid and by the pollination of immature florets. A low frequency of seed was set on both the wheat and the triticale genotypes, having mean seed sets of 0.20 per cent and 0.27 per cent respectively. Although the frequency of embryos (‘seed quality’) was high, doubled haploid production was further limited by poor embryo differentiation and regeneration. Haploid plantlets were obtained from the wheat cultivars Moulin and Renard, although successful chromosome doubling and doubled haploid production was achieved in Moulin only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028617
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Factors affecting haploid production in wheat using the Hordeum bulbosum system. 3. Post-fertilization effects on embryo survival (1987)
    Springer
    Euphytica 36 (1987), S. 763-773 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; frequency of fertilization ; seed set ; seed survival ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An investigation was made of factors affecting the frequency of development of fertilized ovules following wheat x Hordeum bulbosum hybridizations. Seed survival, a term used here to represent the relationship between seed set and fertilization, was shown to vary between the wheat genotypes tested, but was unrelated to the crossability genes present within the wheat genotype. A high seed survival was obtained in crosses involving the variety TH3929 and the single chromosome substitution line, Chinese Spring (Hope 5B). Chinese Spring showed a poor seed survival. Some influence of the H. bulbosum genotype on seed survival was detected. The two environmental factors studied, namely the post-pollination application of gibberellic acid and the ambient temperature, were both shown to affect seed survival. The application of gibberellic acid immediately after pollination or 1 and 2 days after pollination improved seed survival, although the extent of the response was dependent upon the number and timing of the application(s). A genotype-dependent response to the ambient temperature was observed, TH3929 showing a slight but insignificant reduction and Chinese Spring a significant improvement in seed survival with an increase in temperature from 20°C to 26°C. The physiological processes which may be influenced by GA and the ambient temperature and may thereby affect seed survival are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00051859
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    Paper (German National Licenses)
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