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  • Self-incompatibility  (3)
  • Bee pollination  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 1 (1988), S. 25-27 
    ISSN: 1432-2145
    Keywords: Self-incompatibility ; Gramineae ; Two loci system ; Phalaris coerulescens ; Stigma proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protein extracts from four self-incompatible genotypes of Phalaris coerulescens were subjected to analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and ultra-thin isoelectric focusing. A comparison between stigma, root and leaf extracts showed that there was no significant difference in electrophoretic or isoelectric focusing patterns between the genotypes for both root and leaf proteins. However, stigma protein patterns did vary between genotypes especially within the molecular weight region of 43 000–97 000 and within the pI range 5–7. The stigma-specific changes strongly suggest a link between the self-incompatible genotype and these stigma proteins. However, because there are two loci involved, it is not yet possible to precisely assign particular proteins to each S- or Z-allele.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 9 (1996), S. 367-369 
    ISSN: 1432-2145
    Keywords: Key words Gene flow ; Bee pollination ; Almond ; Cross-pollination ; Enpollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Honeybee-assisted gene flow by pollen is of enormous benefit to world food production. Molecular genetic markers were used to detect the pollen-contributing parent in embryos of seeds after fruit set and, subsequently, to deduce the direction and distance of gene flow by pollen as assisted by bees in an almond orchard. It was shown that gene flow by pollen resulting in nut set takes place most strongly between neighbouring sides of adjacent cross-compatible pairs of trees. Examination of pellets extracted from pollen traps fitted to hives for the isozyme markers showed that most of the pellets were composed of pollen from only one cultivar or another, in accord with the hypothesis that the honeybee predominantly visits only one cultivar, either single trees or along rows of one cultivar, and that cross-pollination results from accidental or rare visits involving two cultivars. The results suggest that orchards should be designed to bring cross-compatible pairs of cultivars as close together as possible.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 3 (1990), S. 207-212 
    ISSN: 1432-2145
    Keywords: Self-incompatibility ; Pollen ; Stigma ; Pistil ; Rejection ; Bioassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Self-incompatibility is a form of plant growth regulation acting on pollen and the pollen tube. It could therefore be amenable to study by bioassay techniques, which have been used successfully in the past to show the existence of other plant growth regulators and to assist in their purification. The genetics of self-incompatibility is well understood, and yet there have been difficulties in applying bioassays to the study of the supposed gene products. This review examines published accounts of attempts made to use the bioassay technique in the study of self-incompatibility. In general, bioassays for sporophytic incompatibility have been more successful than gametophytic tests, but none is entirely convincing. Despite this, the authors believe it is worthwhile for those involved with fertilization incompatibility research to persist in trying to improve the bioassay for use as an analytical tool.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 9 (1996), S. 367-369 
    ISSN: 1432-2145
    Keywords: Gene flow ; Bee pollination ; Almond ; Cross-pollination ; Enpollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Honeybee-assisted gene flow by pollen is of enormous benefit to world food production. Molecular genetic markers were used to detect the pollen-contributing parent in embryos of seeds after fruit set and, subsequently, to deduce the direction and distance of gene flow by pollen as assisted by bees in an almond orchard. It was shown that gene flow by pollen resulting in nut set takes place most strongly between neighbouring sides of adjacent cross-compatible pairs of trees. Examination of pellets extracted from pollen traps fitted to hives for the isozyme markers showed that most of the pellets were composed of pollen from only one cultivar or another, in accord with the hypothesis that the honeybee predominantly visits only one cultivar, either single trees or along rows of one cultivar, and that cross-pollination results from accidental or rare visits involving two cultivars. The results suggest that orchards should be designed to bring cross-compatible pairs of cultivars as close together as possible.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 71 (1986), S. 815-819 
    ISSN: 1432-2242
    Keywords: Self-incompatibility ; Petunia hybrida ; Salleles ; Pistil proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Proteins extracted from the pistils of several clones of Petunia hybrida carrying differing pairs of S alleles were examined by gel electrophoresis. The major protein of pistils, a basic glycoprotein of relatively low molecular weight, showed properties which varied in a simple manner with the S genotype. For each S allele we were able to assign a specific molecular weight (ranging from 27,000 to 33,000) and isoelectric point (in the range 8.3 to 8.7) for this putative S protein. Pistils homozygous at the S locus showed only one major protein on two-dimensional gel electrophoresis, while pistils from plants heterozygous at the S locus showed two. No evidence was obtained for the presence of this putative S protein in pollen extracts.
    Type of Medium: Electronic Resource
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