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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 43-45 
    ISSN: 1570-7458
    Keywords: electrical penetration graph ; EPG ; Thrips tabaci ; Frankliniella occidentalis ; cucumber ; leek
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1570-7458
    Keywords: computer simulation model ; population growth ; host-plant resistance ; resistance evaluation ; greenhouse whitefly ; Trialeurodes vaporariorum ; sweetpotato whitefly ; Bemisia tabaci ; Homoptera ; Aleyrodidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract SARAH (Software for theAssessment of antibioticResistance toAleyrodidae inHost plants) is a deterministic simulation model of whitefly population growth based on whitefly life-history components determined on individual plants. The life-history components recorded were oviposition rate, adult survival, pre-adult survival, developmental period, and sex ratio. The simulation model serves as a tool to combine these components and to obtain a single criterion for (antibiotic) resistance. The criterion used was the decrease in simulated intrinsic population growth rate, r s , relative the r s value determined on a susceptible control genotype. This model-based evaluation method was tested using the greenhouse whitefly,Trialeurodes vaporariorum Westwood, on tomato and the sweetpotato whitefly,Bemisia tabaci Gennadius, on tomato, eggplant, collard, and pepper. To study its consistency over time, the evaluation method was repeated six times forT. vaporariorum on a susceptible and a resistant tomato cultivar. Simulated intrinsic population growth rate was more consistent in indicating resistance than any of the individual life-history components. Of tenL. hirsutum accessions tested for resistance toT. vaporariorum, three exhibited r s values that were significantly lower than those for the susceptible control. In addition, on these tenL. hirsutum accessions, a significant positive correlation was observed between r s and sex ratio (# females/# males). Four host plant species (tomato, collard, eggplant, and pepper) were evaluated for resistance toB. tabaci. All life-history components and r s values varied among host species, while a negative r s value was observed forB. tabaci on pepper. A high correlation was found between results from a sensitivity analysis of SARAH and results from a sensitivity analysis of a validated whitefly population simulation model by Yanoet al. (1989a). Significant correlations were found for the relationships between oviposition rate, adult survival, or pre-adult survival and r s , indicating that none of these life-history components can be omitted from the test procedure. This model-based evaluation method offers a standardized way to quantify levels of antibiotic resistance to whiteflies and will enhance efficiency in breeding programs.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 79 (1996), S. 345-353 
    ISSN: 1570-7458
    Keywords: Thysanoptera ; Frankliniella occidentalis ; feeding ; electronic monitoring ; EPG ; cucumber ; chrysanthemum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant penetration by western flower thrips (Frankliniella occidentalis (Pergande)) was analysed with the electrical penetration graph technique (EPG, DC-system). Thrips attached to a gold wire were included in an electrical circuit to record EPGs when penetrating the plant tissues with their stylets. Three basic EPG waveforms have been distinguished, correlated with stylet penetration into cells, salivation, and ingestion, respectively. The main difference with EPGs of Homoptera is the occurrence of continued separate penetrations that are not necessarily followed by ingestion. Insertion of the stylets causes strong voltage fluctuations in the EPG. We could confirm earlier evidence that penetration of cells and subsequent ingestion of (part of) the protoplast takes less than 20 seconds. Repeated short penetrations can be followed by a continuous feeding pattern during which the stylets are not withdrawn. The same sequence of waveforms is produced on other plant parts such as fruits or pollen grains. The specific waveforms are mainly caused by electromotive force (emf). The emf component was recorded with high resolution and the correlation of waveform details with activities of the cibarial muscle system is discussed.
    Type of Medium: Electronic Resource
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