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  • 1
    Electronic Resource
    Electronic Resource
    Platelet factor 4 regulates osteoclastic bone resorption in vitro
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 630 (1980), S. 459-462 
    Details
    ISSN: 0304-4165
    Keywords: Bone resorption ; Ca^2^+ release ; Osteoclast ; Parathyroid hormone ; Platelet factor 4
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(80)90295-0
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  • 2
    Electronic Resource
    Electronic Resource
    Coordination of division events in theChlamydomonas cell cycle (1986)
    Springer
    Protoplasma 131 (1986), S. 118-130 
    Details
    ISSN: 1615-6102
    Keywords: DNA inhibition ; Mitosis ; Microtubules ; Phycoplast ; Plant cytokinesis ; Wall deposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary At concentrations that did not affect growth, hydroxyurea and 21-deoxyadenosine inhibited DNA synthesis inChlamydomonas. Evidence that initiation of mitosis is dependent upon completion of DNA replication was provided by the arrest of inhibited cells with undivided nuclei containing undispersed nucleoli. Initiation of cytokinesis is not dependent upon progress of nuclear division since, in arrested cells, cleavage microtubules became deployed in a phycoplast and a cleavage furrow developed fully, until obstructed by the undivided nucleus. Chloroplast constriction and division also continued independently of nuclear division. It is concluded that nuclear division, cytoplasmic cleavage and chloroplast division are in separate sequences of dependent events. This is supported by flexibility of their relative timing in successive divisions, since after the first commitment to divide nuclear division is followed by initiation of cleavage and then chloroplast division, whereas following subsequent commitments these events occur in reverse time order. This flexibility of order indicates changing rates of progress through separate sequences of events. Deposition of wall material was dependent upon the completion of cytokinesis, but this inhibition of wall deposition by incomplete cytokinesis did not extend to other daughters within the same mother cell. These observations are correlated with our earlier data concerning the rate-limiting control points for division and a model for the coordination of division events is presented. The relationships between different plant cell cycles is discussed in view of the findings presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01285034
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  • 3
    Electronic Resource
    Electronic Resource
    Centrin homologues in higher plants are prominently associated with the developing cell plate (1997)
    Springer
    Protoplasma 196 (1997), S. 224-234 
    Details
    ISSN: 1615-6102
    Keywords: Calmodulin ; Cell plate ; Centrin ; Confocal microscopy ; Immunochemistry ; Mitosis ; Plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Centrin and calmodulin are members of the EF-hand calcium-binding superfamily of proteins. In this study we compared localisation and immunoblotting of centrin with calmodulin in several monocot (onion and wheat) and dicot (mung bean andArabidopsis) plants. We confirmed that an anti-calmodulin antibody recognised a 17 kDa protein in all species tested and localises to the cytoplasm, mitotic matrix and with microtubules of the preprophase band and phragmoplast. In contrast, immunoblotting using anti-centrin antibodies shows that plant centrins vary from 17 to 20 kDa. Immunofluorescence microscopy with anti-centrin antibodies revealed only weak centrin immunoreactivity in the cytoplasm, nucleus, nuclear envelope, phragmoplast and mitotic matrix in meristematic cells. There was a slightly more intense perinuclear labelling in large differentiating onion cells and between separating anaphase chromosomes. While centrin is known to localise to the mitotic spindle poles in animal and algal cells, there was no appreciable immunoreactivity at the spindle poles in higher plants. In contrast, there was an intense immunofluorescence signal with anti-centrin antibodies in the developing cell plate. Further characterisation of the cell plate labelling by immunogold electron microscopy shows centrin immunoreactivity was closely associated with vesicles in the cell plate. Our observations suggest that centrin may play a role in cell plate formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279570
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  • 4
    Electronic Resource
    Electronic Resource
    Identification and measurement of methylamines in elasmobranch tissues using proton nuclear magnetic resonance (1H-NMR) spectroscopy (1998)
    Springer
    Journal of comparative physiology 168 (1998), S. 123-131 
    Details
    ISSN: 1432-136X
    Keywords: Key words Methylamines ; Elasmobranchs ; Nuclear magnetic resonance ; Trimethylamine oxide ; Betaine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Methylamines are frequently present in high concentrations in biological samples, but their separation and quantification are difficult. Data presented show that methylamines commonly occurring in biological material can be uniquely identified and quantified by proton nuclear magnetic resonance spectroscopy by recording spectra at both neutral and acid pH. Use of a high sensitivity probe permits this analysis even in the presence of high water concentrations, allowing accurate quantification with minimum preparative technique. The method was tested on tissues of the dogfish. Trimethylamine oxide was found in amounts ranging from 42 mmol kg−1 fresh weight in liver, up to 115 mmol kg−1 fresh weight in heart. Betaine was found to range from 10 mmol kg−1 fresh weight in liver to 49 mmol kg−1 fresh weight in brain. Creatine was not found in heart or liver, but was present in body wall muscle and in brain. Further analysis using high-performance liquid chromatography allowed determination of urea/methylamine ratios, which ranged from 1.9 in liver to 3.7 in body wall muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003600050128
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