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  • Physics  (2)
  • Betulaceae  (1)
  • Tet repressor  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A possible tertiary structure change induced by acrylamide in the DNA-binding domain of the Tn10-encoded Tet repressor. A fluorescence study (1996)
    Springer
    The protein journal 15 (1996), S. 205-218 
    Details
    ISSN: 1573-4943
    Keywords: Acrylamide ; fluorescence quenching ; Tet repressor ; H-t-H motif ; protein-DNA binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A thorough investigation of the acrylamide fluorescence quenching of F75TetR, a mutant of the Tn10-encoded TetR repressor containing a single Trp residue at position 43, was carried out. The Trp-43 residue is located in a helixα-turn-helixα (H-t-H) motif involved in the specific binding of F75TetR to the operator site in specific DNA. Distinct Ranges of acrylamide concentration have been assumed. At acrylamide concentrations below 0.15–0.2 M (a usual range of values in fluorescence quenching studies) the observed limited tertiary structure change induced by acrylamide is consistent with a noncooperative local unfolding of the DNA-binding domain. It is suggested that penetration of the neutral quencher could cause the deletion of a hydrophobic tertiary structure contact, partly involving TrP-43, responsible for the anchoring of the H-t-H motif inside the three-helix protein bundle, characterizing the N-terminal part. Correspondingly, the affinity of the mutant repressor for the operator was shown to decrease substantially (about five orders of magnitude), seemingly losing its specificity. A subsequent phase, up to 0.8 M acrylamide, was observed in which the involved intermediate protein structure is not further perturbed, nor is DNA binding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01887401
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Restriction fragment polymorphisms in the rDNA region among seven species ofAlnus andBetula papyrifera (1989)
    Springer
    Plant and soil 118 (1989), S. 231-240 
    Details
    ISSN: 1573-5036
    Keywords: alder ; Betulaceae ; birch ; in vitro clones ; cluster ; Frankia ; genetic divergence ; RFPs ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A study of restriction fragment polymorphisms of ribosomal DNA among seven actinorhizal species (Alnus spp.) and a non-actinorhizal species (Betula papyrifera Marsh.) of the Betulaceae was conducted, using a simple method for the extraction of high molecular weight restrictable nuclear DNA from leaf tissues of perennial angiosperms and nine restriction endonucleases. rDNA restriction fragments were variable within and among the species studied, and the variation noted was used to calculate the similarities and infer phenetic relationships among these members of the Betulaceae. The results confirmed the taxonomy of alder based on morphological characters, showing a clear clustering of the species ofAlnus sampled in each of the two different subgeneraAlnus andAlnobetula. Within each subgenus, the closely related taxa often classified as subspecies by their similar morphology and their ability to interhybridize, were similarly shown by restriction fragment polymorphisms to be more closely related to each other than to any other taxon. The analysis also suggested that some alder species may not be more divergent fromBetula papyrifera than from other alder species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02232811
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Photocrosslinking of elastomer systems. I. UV light-induced reactions in a blend of EPDM-benzoin derivatives (1979)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 17 (1979), S. 1685-1700 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Photocrosslinking experiments were performed on elastomeric polymer films (EPDM) that contained benzoin derivatives as photosensitive initiators. Infrared (IR) and ultraviolet (UV) spectroscopy investigations were used in conjunction with physical property measurements. The results all together make it possible to discuss the crosslinking mechanism and pose interesting questions about the reactivity of benzoin derivatives in their excited states in relation to their efficiency in the crosslinking process.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1979.170170611
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  • 4
    Electronic Resource
    Electronic Resource
    Photocrosslinking of elastomer systems. II. Ultraviolet light-induced reactions in an EPDM modified by grafting of benzoin derivatives (1980)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 18 (1980), S. 765-779 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Photocrosslinking experiments were performed on elastomeric polymer films (EPDM) previously modified by grafting a photosensitive initiator (benzoin derivative) onto the macromolecular backbone. This modification was performed by a series of Friedel-Crafts reactions and the resulting materials were characterized by infrared (IR) spectroscopy in conjunction with model reactions. After ultraviolet (UV) irradiation the photocrosslinked samples were studied by IR spectroscopy in conjunction with the measurement of physical properties. It apperared that grafting the photosensitizer onto the elastomer considerably enhances the crosslinking reaction even with a limited quantity of grafted benzoin derivative. Moreover, the results obtained suggest that the structure of the resulting networks is strongly dependent on the crosslinking process used; that is, curing the elastomer with peroxide or UV irradiation either of a blend of elastomer and photosensitizer or of the same elastomer modified by grafting the chromophore.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1980.170180301
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