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  • Binding assay  (1)
  • acute obstructive suppurative cholangitis  (1)
  • 1
    ISSN: 1436-0691
    Keywords: acute obstructive suppurative cholangitis ; elderly patients ; diagnosis ; complications ; biliary drainage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Elderly patients with acute obstructive suppurative cholangitis (AOSC) have a very poor prognosis due both to the gradual development of symptoms and to acute exacerbation, resulting in a condition that is both severe and irreversible. In this study, we evaluated the clinical characteristics and effects of biliary drainage in patients with AOSC treated in our department, with special attention being focused on patients aged 70 years or more. Of 21 patients with AOSC, 7 (33.3%) were more than 70 years of age. All 7 had choledocholithiasis. In all patients with choledocholithiasis, in this study. AOSC was observed in 23.3% of those who were more than 70 years of age (7/30). In elderly patients with AOSC, typical symptoms are often absent, and the severity of the disease is difficult to determine based on the results of examination alone. Since early diagnosis of AOSC and appropriate biliary drainage from the onset of AOSC are extremely important, clinical findings should be examined with the utmost care.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1912
    Keywords: Chromaffin cell ; l-[3H]Nicotine ; Nicotinic acetylcholine receptor ; Binding assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To characterize the properties of nicotinic acetylcholine receptors (nAChRs) in autonomic ganglia, we examined l-[3H]nicotine binding to membrane fraction prepared from cultured bovine adrenal chromaffin cells, using a modified filtration method. Binding of l-[3H]nicotine to non-treated glass fiber filters interfered with the detection of specific binding to the membrane fraction. Presoaking glass fiber filters in 3% or higher concentrations of polyethyleneimine (PEI) solution (sixty times higher than earlier used concentration) for at least 5 h could reduce the binding of l-[3H]nicotine to the filters to the background level. Specific l-[3H]nicotine binding to the membrane fraction was detected only when the membrane fraction was prepared in Ca2+- and Mg2+ (EDTA, EGTA and protease inhibitors were added)-free buffer. Specific binding of l-[3H]nicotine was saturable and reversible. Both computer program and Scatchard analysis revealed a single class of high affinity binding sites with an average Kd of 8.9 nM and a Bmax of 42.5 fmol/mg protein. The Hill coefficient was 0.98. In inhibition studies, both cholinergic agonists (carbachol and l-nicotine) and ganglionic agonists (lobeline and 1,1-dimethyl-4-phenylpiperazinium iodide) were much effective in inhibiting l-[3H]nicotine binding, whereas both neuromuscular blocking (α-bungarotoxin and d-tubocurarine) and ganglionic blocking agents were less effective. These results suggest that high affinity nicotinic binding sites on adrenal chromaffin cells are nAChRs of the ganglion-type, which have properties different from nAChRs on the neuromuscular junction but similar to nAChRs in the brain.
    Type of Medium: Electronic Resource
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