ISSN:
0006-3592
Keywords:
bioassay
;
phytotoxicity
;
photosynthesis
;
immobilized membranes
;
oxygen evolution
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The potential of thylakoid membranes immobilized in an albumin-glutaraldehyde crosslinked matrix in a fast bioassay for phytotoxicity measurements in aqueous samples is studied. Free and immobilized preparations are compared for their electron transport activity measured as the initial rate of oxygen evolution with 2,5-cichlorobenzoquinone as the artificial electron acceptor. Immobilized thylakoids were much stable under storage conditions; in the dark, at 4°C, they were fully stable in terms of photosynthetic activity for a period of 200 h. The immobilized membranes were as sensitive as the free thylakoids for the detection of most of the compounds tested (metal cations, sulfite, nitrite, and herbicides), all known as inhibitors of photosynthetic electron transport. In some instances, the immobilized preparations were even more sensitive than the free counterparts. The sensitivity could be further increased by lowering chlorophyll concentration in the assay. The short incubation period required (∼10 to 15 min) and the small volume of the assay (3 mL) suggest that this type of material should be useful in the detection of locations or effluents with phytotoxic character. © 1994 John Wiley & Sons, Inc.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260440206
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