ISSN:
0006-3592
Keywords:
human albumin
;
Protein conjugate
;
caionic polymer
;
PEG chains
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Human albumin has been attached to 24-hole polystyrene plates via branched poly(ethylene lycol) (PEG) spacer arms. A tetraepoxude of PEG of molecular weight (1.4-1.5) × 104 g/mol was reacted with the protein in solution allowing approximately one-third of the oxirane rings to react. The protein conjugate was then coupled to the long, cationic polymer poly(ethylene imine) (PEI), and the protein-PEG-PEI adduct was subsequently adsorption to unmodified polystyrene. Since the protein is linked to the surface via long, hydrophilic and nonchargedchains, interactions between the biomolecule and the surface is minimized.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260380817
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