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  • Biolistics-GUS-355-Conifer  (1)
  • Transposition  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Dispersed repetitive sequences in the chloroplast genome of Douglas-fir (1989)
    Springer
    Current genetics 16 (1989), S. 211-218 
    Details
    ISSN: 1432-0983
    Keywords: Chloroplast DNA ; Repetitive DNA ; Inversion ; Recombination ; Transposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction mapping and DNA sequencing were used to characterize dispersed repetitive DNA in the chloroplast genome of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. To map repeat families, chloroplast DNA (cpDNA) clones were hybridized at high stringency to one another and to cpDNA cut with restriction enzymes. Repeats are clustered in four regions of the genome and comprise at least six families. Sequence analysis of one repeat family shared among three XbaI fragments indicated the presence of a 633 by inverted repeat which contains a complete tRNA-Serine (GCU) gene and a highly conserved open reading frame (ORF 3.6). Both ends of this 633 bp dispersed repeat have a transposon-like combination of short direct and inverted repeats. One copy of the repeat flanks one of the endpoints of a major inversion which differentiates Douglas-fir from tobacco cpDNA. Dispersion of repetitive DNA by transposition, coupled with loss of the large inverted repeat, appears to have predisposed conifer cpDNA to a number of inversions. An 8 by (CATCTTTT) direct repeat in tobacco is located between two inverted sections in Douglas-fir; it may be a target sequence for homologous recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391479
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transient gene expression of microprojectile-introduced DNA in Douglas-fir cotyledons (1991)
    Springer
    Plant cell reports 10 (1991), S. 517-521 
    Details
    ISSN: 1432-203X
    Keywords: Biolistics-GUS-355-Conifer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasmid DNA containing the reporter gene uidA encoding β-glucuronidase (GUS), driven by the cauliflower mosaic virus 35S promoter, was introduced on high-velocity microprojectiles into cultured cotyledons of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. Transient gene expression was measured by counting the number of distinct loci of GUS activity per cotyledon. Contrary to published results on angiosperms, repeated bombardments did not increase expression in Douglas-fir. Expression varied significantly among cotyledons from different seedlings. The amount of time between DNA delivery and treatment of cotyledons with auxins and cytokinins strongly affected GUS expression. The optimal cytokinin pretreatment produced an average of 20 loci per cotyledon. In several experiments, more than 95% of the treated cotyledons exhibited at least some transient expression. Expression remained constant up to three days following DNA delivery into cotyledons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234585
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    Paper (German National Licenses)
    Fulltext
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