ISSN:
1572-8900
Keywords:
Biopolymer
;
pullulan
;
polysaccharide
;
molecular weight
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Energy, Environment Protection, Nuclear Power Engineering
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract The influence of carbon, nitrogen, and phosphate source, along with concentration, was determined for effect on the weight average molecular weight, molecular weight distribution, and yield of pullulan produced byAureobasidium pullulans NRRL-Y 6220. Batch systems, scale-up batch, and continuous fermentations of 1 L and 10 L were also evaluated as were processing variables, including solvents, and extraction conditions. Products with weight average molecular weight from 1.0 × 105 to 4.0 × 106 were produced in 100-g quantities by varying fermentation conditions such as constituents of the culture medium, pH, and length of incubation. Three sets of culture conditions were defined for the formation of low (〈5.0×105), medium (1.0–2.0×106), and high (〉2.0×106) weight average molecular weight polymer. These defined molecular weight fractions of pullulan were used in further studies in producing films and fibers.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01457648
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