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  • 1
    Electronic Resource
    Electronic Resource
    A direct computer control concept for mammalian cell fermentation processes (1994)
    Springer
    Cytotechnology 15 (1994), S. 271-279 
    Details
    ISSN: 1573-0778
    Keywords: Bioreactor ; control ; computer ; fermentation ; perfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the develoepment of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-acquisition of complex laboratory-scale bioreactors is presented. The fundamental idea was the usage of a standard personal computer, which is connected to pumps, valves and sensors via ADA-transformation. The possibility of free programming allowed the development of user-oriented software, especially designed for the far-reaching requirements of a university laboratory in the field of animal cell culture. Control of aeration, pumps, data-acquisition and data-storage are combined within one program, which allows the automation of standard operations like measurement of kLa- or OTR-values. Pump control algorithms for all common fermentation strategies (batch, fed batch, chemostat, perfusion) are included and can be selected any time during cultivation. Oxygen partial pressure and pH are controlled via direct digital control (ddc), providing simple adaption of control parameters and set points to current fermentation conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00762402
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  • 2
    Electronic Resource
    Electronic Resource
    Scale-up of bioreactors for fermentation of mammalian cell cultures with special reference to oxygen supply and microcarrier mixingPaper presentet by J. Vorlop at the “Jahrestreffen der Verfahrensingenieure”, September 17 to 19, 1986 in Strasbourg (1988)
    Weinheim : Wiley-Blackwell
    Chemical Engineering & Technology - CET 11 (1988), S. 171-178 
    Details
    ISSN: 0930-7516
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The increasing demand for products from mammalian cells has prompted the authors to develop a new type of bioreactor. Its significant features include the supply of oxygen, homogeneous distribution of microcarrier suspensions and process control. Media with high protein contents, required for mammalian cell cultures tend to generate foam. This causes the flotation of solid particles. The reactor was equipped with a system of porous hydrophobic Accurel hollow fibre membranes in order to prevent the formation of bubbles. The membrane is coiled in the form of a basket, or fixed on several carriers. If the liquid pressure is higher than that of the gas phase inside the membrane, a bubble - free oxygen supply to the culture broth can be achieved. The problem of axial mixing of microcarier suspensions was solved by the use of a spiral agitator, attached underneath the aeration system at the bottom of the reactor. The combined aeration and mixing system, which is driven by an eccentric motor, undergoes a tumbling motion. Sufficiently homogeneous suspensions are produced in this system at low membrane velocities, i.e. in presence of low shear forces.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ceat.270110123
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Production of recombinant human antithrombin III on 20-L bioreactor scale: Correlation of supernatant neuraminidase activity, desialylation, and decrease of biological activity of recombinant glycoprotein (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 441-448 
    Details
    ISSN: 0006-3592
    Keywords: Chinese hamster ovary (CHO) cells ; glycoprotein ; recombinant human antithrombin III (rhAT III) ; neuraminidase activity ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Chinese hamster ovary (CHO) cells producing the recombinant glycoprotein human antithrombin III (rhAT III) were batch cultivated in a 20-L bioreactor for 13 days. Neuraminidase activity in cell-free supernatant was monitored during cultivation and free sialic acid was determined by HPLC. Neu5Acα(2→3)Gal-specific Maackia amurensis and Galβ(1→4)GlcNAc-specific Datura stramonium agglutinin were used for determination of sialylated and desialylated rhAT III, respectively. A commercial test kit was used for evaluation of functional rhAT III activity. Supernatant neuraminidase as well as lactate dehydrogenase activity increased significantly during batch growth. The enhanced number of dead cells correlated with increased neuraminidase activity, which seemed to be principally due to cell lysis, resulting in release of cytosolic neuraminidase. Loss of terminally α(2→3) linked sialic acids of the oligosaccharide portions of rhAT III, analyzed in lectin-based Western blot and lectin-adsorbent assays, correlated with a decrease of activity of rhAT III produced throughout long-term batch cultivation. Thus, structural oligosaccharide integrity as well as the functional activity of recombinant glycoprotein depend on the viability and mortality of the bioreactor culture, and batches with a high number of viable cells are required to guarantee production of glycoproteins with maximum biological activity. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 441-448, 1997.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 4
    Electronic Resource
    Electronic Resource
    Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 55 (1997), S. 793-797 
    Details
    ISSN: 0006-3592
    Keywords: repeated batch ; CHO ; cell size ; cell synchronization ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during the lag phase. A prolongation of the stationary phase causes to progressive limitation in asparagine, serine, and ethanolamine. Such extended limitation influences the duration of the following lag phase and obviously induces a synchronization of the cell population that can be monitored easily by a fast cell size analyzing technique. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 793-797, 1997.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 5
    Electronic Resource
    Electronic Resource
    Ein Zellkulturfermenter mit integriertem Membransystem für die homogene KulturführungVortrag von H. Büntemeyer auf dem Jahrestreffen der Verfahrens-Ingenieure, 27. bis 29. Sept. 1989 in Berlin. (1990)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 62 (1990), S. 393-395 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330620508
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  • 6
    Electronic Resource
    Electronic Resource
    Produktverlustfreie Hochtemperatur-Kurzzeitsterilisation von genetisch manipuliertem Escherichia coli (1987)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 59 (1987), S. 577-579 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330590709
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  • 7
    Electronic Resource
    Electronic Resource
    Untersuchungen zur Lösungsstruktur und den rheologischen Eigenschaften von Xanthan während der Fermentation (1986)
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 58 (1986), S. 967-969 
    Details
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cite.330581210
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    Paper (German National Licenses)
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