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  • 1
    Electronic Resource
    Electronic Resource
    Vergleich von Blutbild und Phagocytose nach intravenöser Gabe heterologer Antimakrophagen- oder Antithymuslymphocytenseren (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 325-333 
    Details
    ISSN: 1433-8580
    Keywords: Antimacrophagenserum ; Antithymuslymphocytenserum ; Haemogram ; Phagocytosis ; Antimakrophagenserum ; Antithymuslymphocytenserum ; Blutbild ; Phagocytose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Diein vivo-Wirksamkeit von Kaninchenantiserum gegen Rattenmakrophagen (KARMS) bzw. Rattenthymuslymphocyten (KARTS) wurde nach serologisch erschöpfender Absorption mit Rattenthymuslymphocyten bzw.-makrophagen untersucht. Beide Antiseren waren im Gegensatz zu Kaninchennormalserum stark, jedoch völlig unterschiedlich wirksam. Im peripheren Blut erzeugte KARMS eine selektive 〈 24 Std anhaltende Monocytopenie; KARTS erzeugte eine entsprechende Lymphopenie. Phagocytierende Leberzellen zeigten 3 Std nach i.v. Seruminjektion folgende Veränderungen: KARMS reduzierte die Zahl Kupfferscher Sternzellen, steigerte jedoch stark die Aktivität der vorhandenen, die Monocyten und Granulocyten phagocytierten. KARTS induzierte bei normaler Zahl von Kupffer-Zellen mittlere Aktivitätssteigerung und Phagocytose von Lymphocyten. Diese Befunde werden diskutiert im Rahmen unserer Untersuchungsreihe über Reaktionsspezifitäten heterologer Antiseren nach erschöpfenden Absorptionen mit verschiedenen, kreuzreagierenden Zellarten.
    Notes: Summary In vivo effects of rabbit antisera against rat macrophages (AMS) resp. rat thymic lymphocytes (ALS) were studied after their serologically exhaustive absorption with rat lymphocytes resp. macrophages. In contrast to normal rabbit serum both antisera were strongly, but differently active. In peripheral blood, AMS induced a selective monocytopenia of 〈 24 hrs duration; ALS induced a selective lymphopenia. 3 hrs after i.v. injection of antiserum phagocytosing liver cells showed the following alterations: AMS reduced the number of intact Kupffer cells, but increased the activity of cells still present which phagocytosed monocytes and granulocytes. ALS, on the other hand, did not reduce the number of Kupffer cells but induced phagocytosis of lymphocytes. These results were discussed within the framework of our studies on specificity of reactions of heterologous antisera after their exhaustive absorption with different, cross-reacting cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851519
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  • 2
    Electronic Resource
    Electronic Resource
    Gaft-versus-leukemia activity after bone marrow transplantation does not require graft-versus-host disease (1992)
    Springer
    Annals of hematology 64 (1992), S. 255-259 
    Details
    ISSN: 1432-0584
    Keywords: Bone marrow transplantation ; Leukemia ; Graft-vs-host reaction ; Animal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Clinical data have suggested that graft-versus-host disease (GVHD) plays a crucial role in the antileukemic effects of bone marrow grafts. We investigated (a) whether bone marrow cells unable to induce GVHD can effect graft-versus-leukemia (GVL) activity and (b) whether such antileukemic capacity depends on the presence of T lymphocytes in the graft. Balb/c mice were inoculated with A 20 cells, a B-cell lymphoma/leukemia of Balb/c origin. Four weeks after tumor inoculation the animals were lethally irradiated and received a bone marrow graft. Cells from (Balb/c × C57) F1 or (C3H × Balb/c) F1 hybrids were transplanted into parental-strain Balb/c mice. Since lymphocytes from F1 hybrids are unable to cause graft-versus-host reactivity against a parental-strain animal, we used this experimental setting to explore GVL effects in a GVHD-free system. In vitro incubation with monoclonal anti-Thy-1.2 antibody plus complement was used to eliminate Thy-1+ cells. After syngeneic transplantation, the death rate due to leukemia remained unchanged (91%) compared with that among untreated animals (86%). Following transplantation of F1 marrow cells of either (C57 × Balb/c) F1 or (C3 H × Balb/c) F1 origin, death rates of 40% and 50% were observed; these were significantly lower. Depletion of Thy 1+ cells from bone marrow graft caused only a slight increase in the leukemic death rate after transplantation of bone marrow of (C57 × Balb/c) F1 hybrid origin (50%), but a high leukemic death rate was seen after transplantation of (C3H × Balb/c) F1 bone marrow (100%). Additional experiments with fully allogeneic, T-cell-depleted C57 bone marrow transplantation suggest an antileukemic effect that is comparable to that seen after transplantation of unmanipulated F1 bone marrow. Taken together, our results indicate that GVL activity can be dissociated from graft-versus-host reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01695466
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  • 3
    Electronic Resource
    Electronic Resource
    Patterns of antibody reactivity against selected human leukemia cell lines (1981)
    Springer
    Journal of cancer research and clinical oncology 101 (1981), S. 101-107 
    Details
    ISSN: 1432-1335
    Keywords: ALS ; Bone marrow transplantation ; Cell lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Absorption procedures which allow the production of a selectively cytotoxic anti-human lymphocyte serum are described. Although the production of a reagent whose reactivity is restricted exclusively to lymphocytes may be achieved by exhaustive absorption steps using fresh human erythrocytes, CML cells, and fetal liver cells, a more realistic alternative is the use of appropriately selected cultured human leukemia cell lines. Data are presented which show how these cell lines may be employed to selectively manipulate the crossreactivity spectrum of ALS. Pre-treatment of donor bone marrow cells prior to transplantation with a selectively lymphocytotoxic ALS has been shown to allow transplantation of bone marrow across major histocompatibility barriers in rodents without the occurrence of GvH reactions, and it is the purpose of the present investigations to show that an analogous anti-human ALS can be prepared which possesses the required degree of selectivity to allow its application for human bone marrow transplantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405070
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  • 4
    Electronic Resource
    Electronic Resource
    Ifosfamide and ACNU in experimental allogeneic bone marrow transplantation (1991)
    Springer
    Journal of cancer research and clinical oncology 117 (1991), S. S224 
    Details
    ISSN: 1432-1335
    Keywords: Bone marrow transplantation ; Ifosfamide ; Cyclophosphamide ; ACNU ; Busulfan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have tested ifosfamide and ACNU for their effectiveness in preventing graft rejection following allogeneic bone marrow transplantation. The engraftment-promoting potency of both was compared to that of the standard agent cyclophosphamide. LEW rats received a lethal dose (35 mg/kg) of busulfan followed by injection of 1×108 (CAP×LEW) F1 marrow cells, which are unable to induce a graft vs host reaction in LEW recipients. Rejection of the marrow graft was assessed by monitoring haematocrit and granulocyte count either until death of the animal or until day 80. Surviving animals received a donor-type skin graft to confirm the persistence of allogeneic haematopoiesis. Because of its weak immunosuppressive properties, busulfan by itself is unable to allow engraftment of allogeneic marrow. Therefore, ifosfamide and ACNU and cyclophosphamide as the standard agent could be tested for their capacity to prevent marrow graft rejection. The following rejection rates were observed: cyclophosphamide: 30 mg/ kg 100%, 60 mg/kg 60%, 90 mg/kg 20%, 120 mg/kg and 180 mg/kg 0%; ACNU: 3, 5, 7, and 10 mg/kg 100%, 15 mg/kg 45%, 20 and 30 mg/kg 0%; ifosfamide: 60–120 mg/kg 100%, 180 mg/kg 68%, 240 and 360 mg/kg 0%. Thus, 240 mg/kg ifosfamide or 20 mg/kg ACNU is nearly equivalent to the standard dose of 120 mg/kg cyclophosphamide in engraftment-promoting potency in allogeneic bone marrow transplantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01613232
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