ISSN:
1435-5604
Keywords:
Bone remodeling
;
Bone morphogenetic protein
;
Ectopic bone
;
Osteoclast
;
Acid solution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Bone morphogenetic protein (BMP) from an osteosarcoma powder was found to be solubilized in weak acid (pH 2.6–3.0), but not in acidic solutions of a lower or higher pH. This indicates that the solubility of BMP strictly depends on the hydrogen ion concentration of the solution. Ectopic osteogenesis induced by the osteosarcoma powder was greatly enhanced by treating the powder with acid solutions below pH 3.0. The acid-treated preparations were neutralized before bioassay. Acid treatment resulted in almost a five-fold increase in the amount of new bone formation. This finding suggests that a high concentration of hydrogen ion is important in regulating the activity of BMP. Lyophilized cultured cells of the osteosarcoma preserved the osteogenic activity even after incubation in acidic and neutral buffers at 37°C for six days. This observation suggests that BMP is stable to endogenous enzymes such as lysosomal or proteolytic enzymes. Based on these results, the role possibly played by BMP in the regulation of bone remodeling is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02377311
Permalink
