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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 14 (1988), S. 583-590 
    ISSN: 1432-0983
    Keywords: tRNA genes ; Genetic code ; Mitochondria ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Only three tRNA genes are present within a sequenced 12.35 kbp region of the 15.8 kbp mtDNA of Chlamydomonas reinhardtii, a unicellular green alga. The corresponding tRNAs, whose anticodons are specific for TGG (Trp), CAA/G (Gln) and ATG (Met) codons, all display conventional secondary structures. The tRNAMet gene encodes an elongator rather than initiator species. The standard genetic code is used in C. reinhardtii mitochondria, but codon distribution is highly biased: in a collection of six identified protein coding genes, nine codons (including TGA) are not used at all, while four other sense codons occur very infrequently. In spite of the absence of certain codons, a minimum of 23 tRNAs (assuming separate initiator and elongator tRNAsMet are used) is needed to translate the C. reinhardtii mitochondrial genetic code. It appears unlikely that this minimal tRNA set is encoded by C. reinhardtii mtDNA.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 19 (1991), S. 309-312 
    ISSN: 1432-0983
    Keywords: Chlamydomonas reinhardtii ; Mitochondria ; Repeats ; Spacers ; Processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the mtDNA of Chlamydomonas reinhardtii, a unicellular green alga, we have identified a set of short repeated sequences up to 65 nucleotides long, each of which contains the palindromic consensus motif CTCGG(N4–14)CCGAG. Most of these repeated elements are localized in spacer regions that flank the transcribed coding regions of C. reinhardtii mtDNA. These algal mitochondrial repeats have features reminiscent of short repeats in some fungal mtDNAs, such as GC clusters in Saccharomyces cerevisiae and PstI palindromes in Neurospora crassa. The location of these elements suggests that they could play a role in gene expression, e.g., post-transcriptional processing, in c. reinhardtii mitochondria.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 28 (1988), S. 98-112 
    ISSN: 1432-1432
    Keywords: rRNA ; Evolution ; Sequence comparison ; Parsimony ; Bootstrap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sequences of small subunit (SSU) and large subunit (LSU) ribosomal RNA genes from archaebacteria, eubacteria, and the nucleus, chloroplasts, and mitochondria of eukaryotes have been compared in order to identify the most conservative positions. Aligned sets of these positions for both SSU and LSU rRNA have been used to generate tree diagrams relating the source organisms/organelles. Branching patterns were evaluated using the statistical bootstrapping technique. The resulting SSU and LSU trees are remarkably congruent and show a high degree of similarity with those based on alternative data sets and/or generated by different techniques. In addition to providing insights into the evolution of prokaryotic and eukaryotic (nuclear) lineages, the analysis reported here provides, for the first time, an extensive phylogeny of the mitochondrial lineage.
    Type of Medium: Electronic Resource
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