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  • Bothrops neuwiedi  (1)
  • Composition of cell envelope  (1)
  • Congenital malformation of the hand  (1)
Materialart
Erscheinungszeitraum
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 123 (1979), S. 287-293 
    ISSN: 1432-072X
    Schlagwort(e): Rhodospirillum tenue ; Composition of cell envelope ; Lipids ; Respiratory activities
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Envelope preparations of chemotrophically and phototrophically grown Rhodospirillum tenue were isolated and characterized on the basis of their contents and composition in phospholipids and fatty acids, as well as on the basis of their enzyme activities, absorption spectra and polypeptide patterns. Both preparations were similar in their contents in phospholipids and fatty acids. Their total fatty acids were characterized by a rather high percentage of saturated fatty acids. The activities of the respiratory reactions were considerably higher in chemotrophic than in phototrophic membranes. This is true especially for activities of the terminal oxidase which were over 20 times greater. The affinities of the corresponding enzymes to their respective substrates (K m ) differed with differences in the culture conditions. Under chemotrophic conditions the K m values for the NADH-dependent reactions were lower than those values under phototrophic conditions, whereas the K m values for the succinate dependent reactions were higher. The low temperature (77°K) near infrared spectrum of the phototrophic membrane showed a peak at 875 nm which was not detectable in the chemotrophic membrane. The polypeptide patterns, in the presence of sodium dodecyl sulfate, of both preparations were quite similar except for the presence of two low molecular weight proteins (M. Wt. 12,000 and 10,000) in the phototrophic membrane which were absent in the chemotrophic membrane. Both envelope preparations were further fractionated into enriched cytoplasmic membrane and outer membrane fractions which were identified on the basis of their contents in 2-keto-3-deoxyoctonate, ubiquinone 8 and bacteriochlorophyll (in the case of the phototrophic membrane) and their enzyme activities. The buoyant densities of the corresponding fractions from both envelope preparations were found to be equal. The data presented in this paper demonstrate that envelope preparations of chemotrophically and phototrophically grown R. tenue are similar in their contents in 2-keto-3-deoxyoctonate, ubiquinone 8, phospholipids and fatty acids, yet differ significantly in their spectra, protein patterns and enzyme activities.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-4943
    Schlagwort(e): Bradykinin ; BPPs ; Bothrops neuwiedi ; venom
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract In the course of systematic investigations on low-molecular-weight compounds from the venom of Crotalidae and Viperidae, we have isolated and characterized at least three bradykinin-potentiating peptides (BPP-II, BPP-III, and BPP-V) from Bothrops neuwiedi venom by gel filtration on Sephadex G-25 M, Sephadex G-10 followed by HPLC. The peptides showed bradykinin-potentiating action on isolated guinea-pig ileum, for which the BPP-V was more active than of BPP-II, and BPP-III, rat arterial blood pressure, and a relevant angiotensin-converting enzyme (ACE) competitive inhibiting activity. The kinetic studies showed a K i of the order of 9.7 × 10−3 μM to BPP-II, 7 × 10−3 μM to BPP-III, and 3.3 ×10−3 μM to BPP-V. The amino acid sequence of the BPP-III has been determined to be pGlu-Gly-Gly-Trp-Pro-Arg-Pro-Gly-Pro-Glu-Ile-Pro-Pro, and the amino acid compositions of the BPP-II and BPP-V by amino acid analysis were 2Glu-2Gly-1Arg-4Pro-lIle and 2Glu-2Gly-lSer-3Pro-2Val-Ille, with molecular weight of 1372, 1046, and 1078, respectively.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    European journal of plastic surgery 8 (1984), S. 1-18 
    ISSN: 1435-0130
    Schlagwort(e): Congenital malformation of the hand ; Classification ; Morphogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary At the present time there exist two internationally accepted classifications for the grouping of malformations of extremities: The anatomically and clinically orientated classification by Swanson (1976) and the anatomically and genetically founded classification by Temtamy (1966) and Temtamy and McKusick (1969 and 1978). In the introduction, the phylogeny and ontogeny of the extremities are considered. Under the aspect of phylogeny and ontogeny the groups I Failure of formation of parts, II Failure of differentiation (separation) of parts, III Duplication and VI Congenital constriction band syndrome of Swanson's (1976) classification are discussed. It is found that this anatomically founded classification is largely confirmed in the use of latest morphogenetic criteria and that only few modifications may be called for. In the light of present-day genetic knowledge the use of new classifications published in the literature, for example that of the cleft hand, appear doubtful, as they are based on mere by clinical criteria.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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