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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Inflammation research 42 (1994), S. 141-145 
    ISSN: 1420-908X
    Schlagwort(e): Bradykinin ; Mucin ; Mucus ; HT29 ; Goblet cell
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract The effect of the inflammatory mediator bradykinin on glycoprotein synthesis and mucin secretion in the human colonic adenocarcinoma cell line HT29–18N2 was examined. Bradykinin, at a threshold of 0.01 μM, accelerated the rate of mucin discharge as assessed by a mucin-specific ELISA. Using immunofluorescence microscopy, a thick meshwork of extracellular mucus was observed over bradykinin-treated monolayers but not mock-treated controls. Morphometric analysis of bradykinin-treated monolayers revealed no decreases in intracellular mucin stores or any other easily discernable morphological alteration. The ability of the cyclooxygenase inhibitors indomethacin and naproxen to decrease the response to bradykinin by approximately 68% indicates the effect is mediated, at least partially, through the generation of prostaglandins. Bradykinin did not alter the rate of incorporation of3H-glucosamine into newly synthesized glycoproteins. Bradykinin-accelerated mucin secretion may be linked to the depletion of intracellular mucin stores in the inflammatory bowel disease ulcerative colitis.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 23 (1997), S. 1577-1587 
    ISSN: 1573-1561
    Schlagwort(e): Coffee ; Ceratitis capitata ; odor ; attractants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract We evaluated attraction of released mature laboratory-cultured Mediterranean fruit flies to different sources of coffee plant odor placed in potted nonfruiting guava trees in outdoor field cages. Volatiles from crushed medium or dark red fruit ofCoffea arabica cv. arabica plants were significantly more attractive than volatiles from cut leaves or stems of such plants, volatiles from less-ripe crushedC. arabica fruit, and volatiles from crushed red fruit ofC. racemosa,C. canephora, orC. dewevari. Volatiles fromC. arabica cv. arabica crushed red fruit were equally attractive as volatiles from crushed red fruit ofC. congensis orC. arabica cv. mundo, cv. bourbon, cv. kents or cv. catura. Volatiles from as little as 2 g of crushed redC. arabica fruit (= 1 fruit) were as attractive as volatiles from 32 g of such fruit, demonstrating sensitivity of the bioassay approach used to a small amount of source material. Odor ofC. arabica red fruit refrigerated for 1–10 days after picking was significantly more attractive than odor of fresh-picked fruit, while odor of a 24-hr water extract of intact redC. arabica fruit was significantly more attractive than odor of 24-hr extracts of such fruit with methanol, methylene chloride, or hexane or 1- or 6-hr extracts with water. Extraction studies suggested that at least some of the volatiles of red coffee fruit attractive to medflies may be polar water-soluble molecules. In our final test, volatiles from crushed redC. arabica fruit trapped on Super Q and eluted with methylene chloride proved just as attractive as volatiles emanating directly from crushed fruit of the same type. Together, our findings define optimal source material and effective handling procedures of source material for future identification of volatile components of coffee fruit attractive to medflies.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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