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  • 1
    Electronic Resource
    Electronic Resource
    Competitiveness and communication for effective inoculation byRhizobium, Bradyrhizobium and vesicular-arbuscular mycorrhiza fungi (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 884-889 
    Details
    ISSN: 1420-9071
    Keywords: Bradyrhizobium ; communication ; competitiveness ; Rhizobium ; vesicular-arbuscular (VA) mycorrhiza fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract After a short summary on the ecology and rhizosphere biology of symbiotic bacteria and vesicular-arbuscular (VA) mycorrhiza fungi and their application as microbial inocula, results on competitiveness and communication are summarized. Stress factors such as high temperature, low soil pH, aluminium concentrations and phytoalexins produced by the host plants were studied withRhizobium leguminosarum bv.phaseoli andRhizobium tropici onPhaseolus beans. Quantitative data for competitiveness were obtained by usinggus + (glucoronidase) labelled strains, which produce blue-coloured nodules. ForPhaseolus-nodulating rhizobia, a group specific DNA probe was also developed, which did not hybridize with more than 20 other common soil and rhizosphere bacteria. Results from several laboratories contributing to knowledge of signal exchange and communication in theRhizobium/Bradyrhizobium legume system are summarized in a new scheme, including also defense reactions at the early stages of legume nodule initiation. Stimulating effects of flavonoids on germination and growth of VA mycorrhiza fungi were also found. A constitutive antifungal compound in pea roots, β-isoxazolinonyl-alanine, was characterized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923474
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  • 2
    Electronic Resource
    Electronic Resource
    Autoantibodies to cartilage collagens in relapsing polychondritis (1993)
    Springer
    Archives of dermatological research 285 (1993), S. 245-249 
    Details
    ISSN: 1432-069X
    Keywords: Relapsing polychondritis ; Autoantibody ; Collagen ; Cartilage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Relapsing polychondritis is a systemic disease associated with a destruction of cartilage in various parts of the body. Sera from six patients with relapsing polychondritis and one patient with microscopic polyarteritis nodosa as well as from six controls were analyzed by immunoblotting and ELISA. All patients had autoantibodies against native collagens II and IX. The serum from one patient showed a strong reaction with all three collagen chains of the high molecular weight fraction of collagen IX after denaturation; sera from four patients showed autoantibodies against α2 (XI) and sera from three patients showed autoantibodies against the covalently cross-linked γ component of collagen XI. The presence of autoantibodies against collagens II, IX, and XI, which form the major fibrillar scaffold in cartilage and mediate the interaction of collagen fibrils and proteoglycan, suggests that autoantibodies against cartilaginous collagen may play a crucial role in the pathogenesis of relapsing polychondritis and microscopic polyarteritis nodosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371591
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Hopanoid lipids in Bradyrhizobium and other plant-associated bacteria and cloning of the Bradyrhizobium japonicum squalene-hopene cyclase gene (1996)
    Springer
    Plant and soil 186 (1996), S. 107-112 
    Details
    ISSN: 1573-5036
    Keywords: Bradyrhizobium ; hopanoids ; nitrogen fixation ; plant-microbe interaction ; Rhizobium ; squalene-hopene cyclase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hopanoid lipids have been discovered recently in a number of nitrogen-fixing soil bacteria and in Bradyrhizobium bacteria which fix nitrogen in association with legume plants. We report here an investigation of the hopanoid content in an additional number of soil bacteria capable of living in close association with plants. Of the strains investigated, hopanoids were discovered in phototrophic, nitrogen-fixing bacteria and in an extended number of Bradyrhizobium strains. Strains in which hopanoids so far have not been found belong to the following genera: Rhizobium, Sinorhizobium, Phyllobacterium, Agrobacterium, and Azoarcus. To address the function of hopanoids in Bradyrhizobium, we cloned the gene coding for a key enzyme of hopanoid biosynthesis, the squalene-hopene cyclase, and expressed the gene in E. coli. The recombinant enzyme catalyzed in vitro the cyclization of squalene to hopanoid derivatives.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035063
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of vigilin in chicken cartilage and bone (1993)
    Springer
    Cell & tissue research 273 (1993), S. 381-389 
    Details
    ISSN: 1432-0878
    Keywords: Vigilin ; Cartilage ; Bone ; Differentiation ; In-situ hybridization ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The expression of vigilin was followed during chick embryonal development by in situ hybridization. Vigilin mRNA is abundantly expressed in tissues of mesenchymal and ectomesenchymal origin. The mesenchymal primordial cells of cartilage and bone did not show any significant, expression of vigilin. As tissue differentiation proceeded, vigilin mRNA levels increased in hyaline cartilage and in both endochondral as well as intramembranous bone. The results suggest that the expression of vigilin mRNA in cartilage- and bone-forming cells chondrocytes and osteobalsts, is dependent on the stage of development and cellular differentiation, although not a unique process of bone formation. Most striking is the correlation of the maximum vigilin mRNA expression in osteoblasts and hypertrophic chondrocytes to periods when cell-specific genes were highly transcribed and substantially translated, e.g., synthesis of procollagen and formation of extracellular matrix in bone and cartilage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312841
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    Paper (German National Licenses)
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